OBJECTIVE: Platelet reactivity may be important in the management of patients with stroke. However, degree of platelet reactivity has not been correlated with Thrombelastography (TEG(®)) parameters in stroke. We sought to detect a correlation between TEG(®) values and clot platelet reactivity in ex vivo clots of stroke patients. METHODS: We collected venous blood from 40 patients with stroke. TEG(®) measurements were carried out and residual clots were fixed in 10% formalin immediately following completion of TEG(®). The formalin specimens were embedded in paraffin blocks, cut at 4 micrometers, and stained with CD 61 (immunohistochemical stain used to detect platelets) with appropriate controls. Under light microscopy, three pathologists blinded to TEG(®) results independently graded CD61 intensity (how aggregated/intense the CD61 stained) into a low and high group, as a proposed measurement representing the platelet reactivity of the clot. We compared pre-tPA-TEG(®) values among groups with different CD 61 intensities. RESULTS: After adjusting for confounding factors, we found statistically significant correlation between CD61 staining and several TEG(®) parameters (Delta and CD61 staining intensity (p=0.047); Angle and CD61 staining intensity grade (p=0.04); and G and CD61 staining intensity grade (p=0.04)). CONCLUSIONS: Clot strength on TEG(®) as measured by Delta, Angle, and G correlates with a clot with greater platelet reactivity.
OBJECTIVE: Platelet reactivity may be important in the management of patients with stroke. However, degree of platelet reactivity has not been correlated with Thrombelastography (TEG(®)) parameters in stroke. We sought to detect a correlation between TEG(®) values and clot platelet reactivity in ex vivo clots of strokepatients. METHODS: We collected venous blood from 40 patients with stroke. TEG(®) measurements were carried out and residual clots were fixed in 10% formalin immediately following completion of TEG(®). The formalin specimens were embedded in paraffin blocks, cut at 4 micrometers, and stained with CD 61 (immunohistochemical stain used to detect platelets) with appropriate controls. Under light microscopy, three pathologists blinded to TEG(®) results independently graded CD61 intensity (how aggregated/intense the CD61 stained) into a low and high group, as a proposed measurement representing the platelet reactivity of the clot. We compared pre-tPA-TEG(®) values among groups with different CD 61 intensities. RESULTS: After adjusting for confounding factors, we found statistically significant correlation between CD61 staining and several TEG(®) parameters (Delta and CD61 staining intensity (p=0.047); Angle and CD61 staining intensity grade (p=0.04); and G and CD61 staining intensity grade (p=0.04)). CONCLUSIONS: Clot strength on TEG(®) as measured by Delta, Angle, and G correlates with a clot with greater platelet reactivity.
Authors: Mark M McDonald; Jeremy Wetzel; Stuart Fraser; Andrea Elliott; Ritvij Bowry; Jorge F Kawano-Castillo; Chunyan Cai; Navdeep Sangha; Jessica Messier; Amanda Hassler; Joancy Archeval-Lao; Stephanie A Parker; Mohammad H Rahbar; Evan G Pivalizza; Tiffany R Chang; James C Grotta Journal: J Thromb Thrombolysis Date: 2016-04 Impact factor: 2.300
Authors: Gregory R Stettler; Ernest E Moore; Geoffrey R Nunns; Marguerite Kelher; Anirban Banerjee; Christopher C Silliman Journal: J Surg Res Date: 2020-12-02 Impact factor: 2.192
Authors: Wilbert L Jones; Christopher R Ramos; Anirban Banerjee; Ernest E Moore; Kirk C Hansen; Julia R Coleman; Marguerite Kelher; Keith B Neeves; Christopher C Silliman; Jorge Di Paola; Brian Branchford Journal: Platelets Date: 2022-06-05 Impact factor: 4.236
Authors: Adam Wiśniewski; Aleksandra Karczmarska-Wódzka; Joanna Sikora; Przemysław Sobczak; Adam Lemanowicz; Karolina Filipska; Robert Ślusarz Journal: Diagnostics (Basel) Date: 2021-04-15