| Literature DB >> 26116468 |
Mengsi Yu1, Huizhen Zhang2, Yiwen Liu1, Yiqing He1, Cuixia Yang1, Yan Du1, Man Wu1, Guoliang Zhang1, Feng Gao3.
Abstract
Lymphangiogenesis, the formation of new lymph vessels, plays a significant role in the development and metastasis of various cancers. We and others have demonstrated that low molecular weight hyaluronan (LMW-HA) promotes lymphangiogenesis. However, the underlying mechanisms are poorly defined. In this study, using immunofluorescence and co-immunoprecipitation, we found that LMW-HA increased the colocalization of lymphatic vessel endothelial HA receptor (LYVE-1) and sphingosine 1-phosphate receptor (S1P3) at the cell surface. Silencing of either LYVE-1 or S1P3 decreased LMW-HA-mediated tube formation in lymphatic endothelial cells (LECs). Furthermore, silencing of either LYVE-1 or S1P3 significantly inhibited LMW-HA-induced tyrosine phosphorylation of Src kinase and extracellular signal-regulated kinase (ERK1/2). In summary, these results suggest that S1P3 and LYVE-1 may cooperate to play a role in LMW-HA-mediated lymphangiogenesis. This interaction may provide a useful target for the intervention of lymphangiogenesis-associated tumor progression.Entities:
Keywords: LMW-HA; LYVE-1; Lymphangiogenesis; S1P3
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Year: 2015 PMID： 26116468 DOI： 10.1016/j.yexcr.2015.06.014
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905