Daniela Palmieri1, Marzia Mura2, Simone Mambrini3, Domenico Palombo2. 1. Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical, Vascular Biology, DISC, University of Genoa, IRCCS San Martino-IST, 16132 Genoa, Italy; Research Center of Biologically Inspired Engineering for Vascular Medicine and Longevity (BELONG), Genoa, Italy. Electronic address: danielapalmieri@yahoo.com. 2. Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical, Vascular Biology, DISC, University of Genoa, IRCCS San Martino-IST, 16132 Genoa, Italy; Research Center of Biologically Inspired Engineering for Vascular Medicine and Longevity (BELONG), Genoa, Italy. 3. Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical, Vascular Biology, DISC, University of Genoa, IRCCS San Martino-IST, 16132 Genoa, Italy.
Abstract
PURPOSE: One of the limitations emerged with both synthetic and degradable vascular grafts is the lack of endothelialization after implantation that is known to be the main reason leading to unfavourable outcomes. It emerges the need to find new strategies to promote a rapid endothelialization of the scaffold. Pleiotrophin is a growth/differentiation cytokine for various cell type. We here evaluated the effect of Pleiotrophin on endothelial cells (EC), monocytes and macrophages that have been shown as key cells promoting neovascularization. MATERIAL/ METHODS: EA.hy926 endothelial cells, THP-1 monocytes and PMA-differentiated macrophages were treated with Pleiotrophin (10 and 100ng/ml). VEGF, Flk-1, Nrp-1, COX-2, ICAM-1 and TGFβ expression were detected by Western Blot, IL-10, MCP-1 and TNFα levels by ELISA. Chemotaxis was performed in Boyden chambers. Wound healing was performed by scratch wound assay. RESULTS: Pleiotrophin induces in EC the expression of VEGF and its receptors Flk-1 and Nrp-1 and improves the migratory capacity. In THP-1 monocytes, Pleiotrophin induces the expression of VEGF and its receptor Nrp-1 and decreases the levels of COX-2 and TNFα. In PMA-differentiated macrophages COX-2 expression was significantly reduced by Pleiotrophin, while IL-10 and TGFβ were increased. CONCLUSIONS: Pleiotrophin acts as an angiogenesis 'driver' by promoting the creation of a pro-angiogenic environment, a migratory behaviour in EC and a pro-regenerative alternative phenotype in macrophages. Our results suggest that Pleiotrophin might be considered for vascular prosthesis engineering.
PURPOSE: One of the limitations emerged with both synthetic and degradable vascular grafts is the lack of endothelialization after implantation that is known to be the main reason leading to unfavourable outcomes. It emerges the need to find new strategies to promote a rapid endothelialization of the scaffold. Pleiotrophin is a growth/differentiation cytokine for various cell type. We here evaluated the effect of Pleiotrophin on endothelial cells (EC), monocytes and macrophages that have been shown as key cells promoting neovascularization. MATERIAL/ METHODS: EA.hy926 endothelial cells, THP-1 monocytes and PMA-differentiated macrophages were treated with Pleiotrophin (10 and 100ng/ml). VEGF, Flk-1, Nrp-1, COX-2, ICAM-1 and TGFβ expression were detected by Western Blot, IL-10, MCP-1 and TNFα levels by ELISA. Chemotaxis was performed in Boyden chambers. Wound healing was performed by scratch wound assay. RESULTS:Pleiotrophin induces in EC the expression of VEGF and its receptors Flk-1 and Nrp-1 and improves the migratory capacity. In THP-1 monocytes, Pleiotrophin induces the expression of VEGF and its receptor Nrp-1 and decreases the levels of COX-2 and TNFα. In PMA-differentiated macrophages COX-2 expression was significantly reduced by Pleiotrophin, while IL-10 and TGFβ were increased. CONCLUSIONS:Pleiotrophin acts as an angiogenesis 'driver' by promoting the creation of a pro-angiogenic environment, a migratory behaviour in EC and a pro-regenerative alternative phenotype in macrophages. Our results suggest that Pleiotrophin might be considered for vascular prosthesis engineering.
Authors: Ming Chen; Yi Li; Xiang Huang; Ya Gu; Shang Li; Pengbin Yin; Licheng Zhang; Peifu Tang Journal: Bone Res Date: 2021-03-22 Impact factor: 13.567
Authors: Ivones Hernández-Balmaseda; Idania Rodeiro Guerra; Ken Declerck; José Alfredo Herrera Isidrón; Claudina Pérez-Novo; Guy Van Camp; Olivier De Wever; Kethia González; Mayrel Labrada; Adriana Carr; Geovanni Dantas-Cassali; Diego Carlos Dos Reis; Livan Delgado-Roche; Roberto Rafael Nuñez; René Delgado-Hernández; Miguel David Fernández; Miriam T Paz-Lopes; Wim Vanden Berghe Journal: Mar Drugs Date: 2021-01-22 Impact factor: 5.118