Eunice Kuhn1, Patrícia Farhat2, Ana Paula Teitelbaum2, Alexandra Mena-Serrano3, Alessandro D Loguercio1, Alessandra Reis4, David H Pashley5. 1. Department of Restorative Dentistry, State University of Ponta Grossa, Rua Carlos Cavalcanti, 4748, Bloco M, Sala 64A - Uvaranas, Ponta Grossa, Paraná, Brazil. 2. School of Dentistry, CESCAGE (Cenro de Ensino Superior dos Campos Gerais), Rua Tomazina S.N - Olarias, Ponta Grossa, Paraná, Brazil. 3. Department of Restorative Dentistry, Universidad de las Americas, Av. de los Granados E12-41, Pichincha, Quito, Ecuador. 4. Department of Restorative Dentistry, State University of Ponta Grossa, Rua Carlos Cavalcanti, 4748, Bloco M, Sala 64A - Uvaranas, Ponta Grossa, Paraná, Brazil. Electronic address: reis_ale@hotmail.com. 5. Department of Oral Biology, Medical College of Georgia, School of Dentistry, 1120 15th Street, CL2112, Augusta, GA, USA.
Abstract
OBJECTIVES: This study evaluated the microtensile bond strength (μTBS) and nanoleakage (NL) of dentin bonded interfaces produced with ethanol-wet and water-wet bonding protocols under clinical and laboratory conditions. METHODS: The sample was composed of forty primary second molars in advanced exfoliation process. Occlusal cavities were prepared leaving a flat dentin surface on the pulpal floor. In half of the teeth, the water-wet protocol was followed using a three-step etch-and-rinse adhesive. In the other half, dentin was dehydrated with ascending ethanol solutions (50%, 70%, 80%, 95% and 3×100%), 15s each for the ethanol-bonding protocol. An experimental hydrophobic primer was used, followed by the neat adhesive application. Resin build-ups were prepared, stored for 24h, sectioned into sticks and tested in tensile mode (0.5mm/min). NL was performed for all groups. The μTBS and NL data were submitted to two-way ANOVA and Kruskall-Wallis tests, respectively (α=0.05). RESULTS: Under clinical conditions, the highest μTBS was observed for the water-wet bonding while under the laboratory setting, the highest μTBS was obtained for the ethanol-wet bonding. Increased NL was observed in the water-wet bonding groups irrespective of the bonding condition. SIGNIFICANCE: The immediate benefits of the ethanol-bonding observed in the laboratory setting was not confirmed when the same protocol was performed in vivo. However, as reduced nanoleakage was seen in adhesive interfaces produced with the ethanol-wet bonding technique, suggests that the hybrid layer may be more resistant to degradation.
OBJECTIVES: This study evaluated the microtensile bond strength (μTBS) and nanoleakage (NL) of dentin bonded interfaces produced with ethanol-wet and water-wet bonding protocols under clinical and laboratory conditions. METHODS: The sample was composed of forty primary second molars in advanced exfoliation process. Occlusal cavities were prepared leaving a flat dentin surface on the pulpal floor. In half of the teeth, the water-wet protocol was followed using a three-step etch-and-rinse adhesive. In the other half, dentin was dehydrated with ascending ethanol solutions (50%, 70%, 80%, 95% and 3×100%), 15s each for the ethanol-bonding protocol. An experimental hydrophobic primer was used, followed by the neat adhesive application. Resin build-ups were prepared, stored for 24h, sectioned into sticks and tested in tensile mode (0.5mm/min). NL was performed for all groups. The μTBS and NL data were submitted to two-way ANOVA and Kruskall-Wallis tests, respectively (α=0.05). RESULTS: Under clinical conditions, the highest μTBS was observed for the water-wet bonding while under the laboratory setting, the highest μTBS was obtained for the ethanol-wet bonding. Increased NL was observed in the water-wet bonding groups irrespective of the bonding condition. SIGNIFICANCE: The immediate benefits of the ethanol-bonding observed in the laboratory setting was not confirmed when the same protocol was performed in vivo. However, as reduced nanoleakage was seen in adhesive interfaces produced with the ethanol-wet bonding technique, suggests that the hybrid layer may be more resistant to degradation.
Authors: Kang Li; Chenmin Yao; Yuhong Sun; Kun Wang; Xiangtao Wang; Zhengzhi Wang; James Kit Hon Tsoi; Cui Huang; Cynthia Kar Yung Yiu Journal: Mater Today Bio Date: 2021-11-29