Sivakamasundari Pichu1, Jayalalitha Sathiyamoorthy2, Ezhilarasi Krishnamoorthy2, Dhamodharan Umapathy2, Vijay Viswanathan3. 1. Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, Royapuram, Chennai 600013, Tamil Nadu, India. Electronic address: au_sudha@yahoo.com. 2. Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, Royapuram, Chennai 600013, Tamil Nadu, India. 3. Prof. M. Viswanathan Diabetes Research Centre, MV Hospital for Diabetes, Royapuram, Chennai 600013, Tamil Nadu, India. Electronic address: dr_vijay@mvdiabetes.com.
Abstract
AIM: Adaptation to low oxygen tension (hypoxia) in cells and tissues leads to the transcriptional induction of series of genes and the primary factor mediating this response is the hypoxia-inducible factor-1α. This study was designed in order to examine the HIF-1α gene polymorphism, p582s (rs11549465) in Exon-12 of HIF-1α gene in diabetic subjects with and without foot ulcers (DFU) and to find its expression under these pathological conditions. METHODS: A total of 224 subjects from our tertiary care centre were included, which consists of healthy controls (N=66), type 2 diabetes mellitus (T2DM) (N=79) and T2DM with foot ulcers (DFU) (N=79). Allelic and genotypic comparisons between the different groups were evaluated by PCR-RFLP. The gene expression studies on selected samples (N=15 of each group) were done by Semi-quantitative real time PCR. RESULTS AND DISCUSSIONS: Genotype analysis showed a significant increase in presence of 'T' allele in T2DM & DFU when compared to that of control subjects. Allele wise analysis showed a higher frequency of 'T' allele in the T2DM (62.03%) when compared to that of control subjects (53.79%). Interestingly, semi-quantitative RT-PCR results showed decreased expression of HIF-1α gene on DFU when compared to that of T2DM and control subjects. CONCLUSION: Our findings predict that there is an association of HIF-1α gene polymorphism on foot ulcer patients when compare to that of healthy controls. Semi-quantitative real time studies showed decreased HIF-1α gene expression on foot ulcer patients suggesting its possible role on the pathogenesis.
AIM: Adaptation to low oxygen tension (hypoxia) in cells and tissues leads to the transcriptional induction of series of genes and the primary factor mediating this response is the hypoxia-inducible factor-1α. This study was designed in order to examine the HIF-1α gene polymorphism, p582s (rs11549465) in Exon-12 of HIF-1α gene in diabetic subjects with and without foot ulcers (DFU) and to find its expression under these pathological conditions. METHODS: A total of 224 subjects from our tertiary care centre were included, which consists of healthy controls (N=66), type 2 diabetes mellitus (T2DM) (N=79) and T2DM with foot ulcers (DFU) (N=79). Allelic and genotypic comparisons between the different groups were evaluated by PCR-RFLP. The gene expression studies on selected samples (N=15 of each group) were done by Semi-quantitative real time PCR. RESULTS AND DISCUSSIONS: Genotype analysis showed a significant increase in presence of 'T' allele in T2DM & DFU when compared to that of control subjects. Allele wise analysis showed a higher frequency of 'T' allele in the T2DM (62.03%) when compared to that of control subjects (53.79%). Interestingly, semi-quantitative RT-PCR results showed decreased expression of HIF-1α gene on DFU when compared to that of T2DM and control subjects. CONCLUSION: Our findings predict that there is an association of HIF-1α gene polymorphism on foot ulcerpatients when compare to that of healthy controls. Semi-quantitative real time studies showed decreased HIF-1α gene expression on foot ulcerpatients suggesting its possible role on the pathogenesis.
Authors: Amit Lalwani; Joanna Warren; David Liuwantara; Wayne J Hawthorne; Philip J O'Connell; Frank J Gonzalez; Rebecca A Stokes; Jennifer Chen; D Ross Laybutt; Maria E Craig; Michael M Swarbrick; Cecile King; Jenny E Gunton Journal: Cell Rep Date: 2019-05-21 Impact factor: 9.423
Authors: Annika Höcker; Miriam Rabeling; Alexandra Bick; Linda Cox; Maximiliane Kreuzer; Andrea Engler; Kai Walstein; Hagen S Bachmann; Karl-Heinz Jöckel; Lewin Eisele; Michael Adamzik; Jürgen Peters; Simon T Schäfer Journal: BMC Anesthesiol Date: 2016-08-11 Impact factor: 2.217