Fangyu Peng1, Otto Muzik2, Joshua Gatson3, Steven G Kernie4, Ramon Diaz-Arrastia5. 1. Department of Radiology, University of Texas Southwestern Medical Center, Dallas, Texas Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, Texas fangyu.peng@utsouthwestern.edu. 2. Carman and Ann Adams Department of Pediatrics, School of Medicine, Wayne State University, Detroit, Michigan Department of Radiology, School of Medicine, Wayne State University, Detroit, Michigan. 3. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas. 4. Department of Pediatrics and Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York; and. 5. Center for Neurosciences and Regenerative Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
Abstract
UNLABELLED: Copper is a nutritional trace element required for cell proliferation and wound repair. METHODS: To explore increased copper uptake as a biomarker for noninvasive assessment of traumatic brain injury (TBI), experimental TBI in C57BL/6 mice was induced by controlled cortical impact, and (64)Cu uptake in the injured cortex was assessed with (64)CuCl2 PET/CT. RESULTS: At 24 h after intravenous injection of the tracer, uptake was significantly higher in the injured cortex of TBI mice (1.15 ± 0.53 percentage injected dose per gram of tissue [%ID/g]) than in the uninjured cortex of mice without TBI (0.53 ± 0.07 %ID/g, P = 0.027) or the cortex of mice that received an intracortical injection of zymosan A (0.62 ± 0.22 %ID/g, P = 0.025). Furthermore, uptake in the traumatized cortex of untreated TBI mice (1.15 ± 0.53 %ID/g) did not significantly differ from that in minocycline-treated TBI mice (0.93 ± 0.30 %ID/g, P = 0.33). CONCLUSION: Overall, the data suggest that increased (64)Cu uptake in traumatized brain tissues holds potential as a new biomarker for noninvasive assessment of TBI with (64)CuCl2 PET/CT.
UNLABELLED: Copper is a nutritional trace element required for cell proliferation and wound repair. METHODS: To explore increased copper uptake as a biomarker for noninvasive assessment of traumatic brain injury (TBI), experimental TBI in C57BL/6 mice was induced by controlled cortical impact, and (64)Cu uptake in the injured cortex was assessed with (64)CuCl2PET/CT. RESULTS: At 24 h after intravenous injection of the tracer, uptake was significantly higher in the injured cortex of TBI mice (1.15 ± 0.53 percentage injected dose per gram of tissue [%ID/g]) than in the uninjured cortex of mice without TBI (0.53 ± 0.07 %ID/g, P = 0.027) or the cortex of mice that received an intracortical injection of zymosan A (0.62 ± 0.22 %ID/g, P = 0.025). Furthermore, uptake in the traumatized cortex of untreated TBI mice (1.15 ± 0.53 %ID/g) did not significantly differ from that in minocycline-treated TBI mice (0.93 ± 0.30 %ID/g, P = 0.33). CONCLUSION: Overall, the data suggest that increased (64)Cu uptake in traumatized brain tissues holds potential as a new biomarker for noninvasive assessment of TBI with (64)CuCl2PET/CT.
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