| Literature DB >> 26111446 |
Michelle L Ratliff1, Rebecca Powell2, Carol F Webb2,1,3, Celeste R Wirsig-Wiechmann2, Olga Lakiza2, Tomoko Obara2.
Abstract
Despite exciting new possibilities for regenerative therapy posed by the ability to induce pluripotent stem cells, recapitulation of three-dimensional kidneys for repair or replacement has not been possible. ARID3a-deficient mouse tissues generated multipotent, developmentally plastic cells. Therefore, we assessed the adult mouse ARID3a-/- kidney cell line, KKPS5, which expresses renal progenitor surface markers as an alternative cell source for modeling kidney development. Remarkably, these cells spontaneously developed into multicellular nephron-like structures in vitro, and engrafted into immunocompromised medaka mesonephros, where they formed mouse nephron structures. These data implicate KKPS5 cells as a new model system for studying kidney development.Entities:
Keywords: ARID3a; Medaka; Nephron structure; Renal progenitor
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Year: 2015 PMID： 26111446 PMCID： PMC4526183 DOI： 10.1016/j.bbrc.2015.06.130
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575