David P Gavin1,2, Handojo Kusumo1,2, Rajiv P Sharma1,2, Marina Guizzetti1,2,3, Alessandro Guidotti2, Subhash C Pandey1,2. 1. Jesse Brown Veterans Affairs Medical Center, 820 South Damen Avenue (M/C 151), Chicago, IL 60612, USA. 2. Department of Psychiatry, University of Illinois at Chicago, 1601 W Taylor St, Chicago, IL 60612, USA. 3. Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, OR and VA Portland Health Care System, Portland, OR.
Abstract
AIM: In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf. MATERIALS & METHODS: Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were treated with N-methyl-d-aspartate (NMDA). Expression changes of genes reportedly involved in DNA demethylation, Bdnf mRNA and protein and 5MC and 5HMC at Bdnf promoters were measured. RESULTS: Gadd45b siRNA transfection in neurons abolishes the NMDA-induced increase in Bdnf IXa mRNA and reductions in 5MC and 5HMC at the Bdnf IXa promoter. CONCLUSION: These results contribute to our understanding of DNA demethylation mechanisms in neurons, and its role in regulating NMDA responsive genes implicated in mental illnesses.
AIM: In nondividing neurons examine the role of Gadd45b in active 5-methylcytosine (5MC) and 5-hydroxymethylcytosine (5HMC) removal at a gene promoter highly implicated in mental illnesses and cognition, Bdnf. MATERIALS & METHODS:Mouse primary cortical neuronal cultures with and without Gadd45b siRNA transfection were treated with N-methyl-d-aspartate (NMDA). Expression changes of genes reportedly involved in DNA demethylation, Bdnf mRNA and protein and 5MC and 5HMC at Bdnf promoters were measured. RESULTS:Gadd45b siRNA transfection in neurons abolishes the NMDA-induced increase in Bdnf IXa mRNA and reductions in 5MC and 5HMC at the Bdnf IXa promoter. CONCLUSION: These results contribute to our understanding of DNA demethylation mechanisms in neurons, and its role in regulating NMDA responsive genes implicated in mental illnesses.
Authors: Shinsuke Ito; Ana C D'Alessio; Olena V Taranova; Kwonho Hong; Lawrence C Sowers; Yi Zhang Journal: Nature Date: 2010-08-26 Impact factor: 49.962
Authors: Mark E Pepin; Concetta Schiano; Marco Miceli; Giuditta Benincasa; Gelsomina Mansueto; Vincenzo Grimaldi; Andrea Soricelli; Adam R Wende; Claudio Napoli Journal: Exp Cell Res Date: 2021-01-27 Impact factor: 3.905
Authors: Manal H Saad; Matthew Rumschlag; Michael H Guerra; Candace L Savonen; Alaina M Jaster; Philip D Olson; Adnan Alazizi; Francesca Luca; Roger Pique-Regi; Carl J Schmidt; Michael J Bannon Journal: Sci Rep Date: 2019-02-07 Impact factor: 4.379
Authors: Katrin M Schüle; Manuel Leichsenring; Tommaso Andreani; Viviana Vastolo; Medhavi Mallick; Michael U Musheev; Emil Karaulanov; Christof Niehrs Journal: Genes Dev Date: 2019-06-06 Impact factor: 11.361
Authors: Morgan E Zipperly; Faraz A Sultan; Guan-En Graham; Andrew C Brane; Natalie A Simpkins; Nancy V N Carullo; Lara Ianov; Jeremy J Day Journal: Neuropsychopharmacology Date: 2020-09-14 Impact factor: 7.853