Ching-Shu Lai1, Sih-Ning Liao2, Mei-Ling Tsai2, Nagabhushanam Kalyanam3, Muhammed Majeed3, Anju Majeed4, Chi-Tang Ho5, Min-Hsiung Pan1,6,7. 1. Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan. 2. Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan. 3. Sabinsa Corporation, East Windsor, NJ, USA. 4. Research and Development, Sami Labs Limited, Bangalore, India. 5. Department of Food Science, Rutgers University, New Brunswick, NJ, USA. 6. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. 7. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
Abstract
SCOPE: Diet-induced obesity and associated nonalcoholic fatty liver disease have increased and become a major health problem worldwide. This study was conducted to investigate the chemopreventive effects of dietary Calebin-A, a curcuminoid, on differentiation of 3T3-L1 adipocytes and high-fat diet (HFD) induced obesity and hepatic steatosis. Potential mechanisms contributing to these effects were also elucidated. METHODS AND RESULTS: Calebin-A effectively and dose dependently suppressed accumulation of lipid droplets in adipocytes through the suppression of adipogenic specific factor peroxisome proliferator-activated receptor (PPAR) γ and fatty acid synthase and activated acetyl-CoA carboxylase. Dietary Calebin-A effectively decreased weight gain and relative perigonadal, retroperitoneal, and mesenteric fat weight in HFD-fed mice. Furthermore, Calebin-A markedly reduced hepatic steatosis and the serum levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, total cholesterol, and triacylglycerol. These effects were associated with the downregulation of PPARγ, sterol regulatory element-binding protein-1, and particularly the activation of AMP-activated protein kinase α signaling found in both adipocytes and liver tissues. CONCLUSION: Taken together, these results demonstrated for the first time that Calebin-A suppressed adipocyte differentiation, prevented HFD-induced obesity, and improved hepatic steatosis, suggesting a novel application for the prevention and treatment of obesity and associated nonalcoholic fatty liver disease.
SCOPE: Diet-induced obesity and associated nonalcoholic fatty liver disease have increased and become a major health problem worldwide. This study was conducted to investigate the chemopreventive effects of dietary Calebin-A, a curcuminoid, on differentiation of 3T3-L1 adipocytes and high-fat diet (HFD) induced obesity and hepatic steatosis. Potential mechanisms contributing to these effects were also elucidated. METHODS AND RESULTS:Calebin-A effectively and dose dependently suppressed accumulation of lipid droplets in adipocytes through the suppression of adipogenic specific factor peroxisome proliferator-activated receptor (PPAR) γ and fatty acid synthase and activated acetyl-CoA carboxylase. Dietary Calebin-A effectively decreased weight gain and relative perigonadal, retroperitoneal, and mesenteric fat weight in HFD-fed mice. Furthermore, Calebin-A markedly reduced hepatic steatosis and the serum levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, total cholesterol, and triacylglycerol. These effects were associated with the downregulation of PPARγ, sterol regulatory element-binding protein-1, and particularly the activation of AMP-activated protein kinase α signaling found in both adipocytes and liver tissues. CONCLUSION: Taken together, these results demonstrated for the first time that Calebin-A suppressed adipocyte differentiation, prevented HFD-induced obesity, and improved hepatic steatosis, suggesting a novel application for the prevention and treatment of obesity and associated nonalcoholic fatty liver disease.
Authors: Cécile Héliès-Toussaint; Edwin Fouché; Nathalie Naud; Florence Blas-Y-Estrada; Maria Del Socorro Santos-Diaz; Anne Nègre-Salvayre; Ana Paulina Barba de la Rosa; Françoise Guéraud Journal: BMC Complement Med Ther Date: 2020-02-05