Literature DB >> 26107907

[An intriguing model for 5S rDNA sequences dispersion in the genome of freshwater stingray Potamotrygon motoro (Chondrichthyes: Potamotrygonidae)].

V P Cruz1,2, C Oliveira1, F Foresti1.   

Abstract

5S rDNA genes of the stingray Potamotrygon motoro were PCR replicated, purified, cloned and sequenced. Two distinct classes of segments of different sizes were obtained. The smallest, with 342 bp units, was classified as class I, and the largest, with 1900 bp units, was designated as class II. Alignment with the consensus sequences for both classes showed changes in a few bases in the 5S rDNA genes. TATA-like sequences were detected in the nontranscribed spacer (NTS) regions of class I and a microsatellite (GCT) 10 sequence was detected in the NTS region of class II. The results obtained can help to understand the molecular organization of ribosomal genes and the mechanism of gene dispersion.

Entities:  

Keywords:  5S rDNA; Elasmobranchii; Potamotrygon; clone; microsatellite; stingray

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Year:  2015        PMID: 26107907     DOI: 10.7868/S0026898415030039

Source DB:  PubMed          Journal:  Mol Biol (Mosk)        ISSN: 0026-8984


  1 in total

1.  Chromosomal characterization of Amazonian freshwater stingrays with evidence for new karyomorphs and XX/XY sex chromosomes.

Authors:  Francisco Carlos de Souza Valentim; Jorge Ivan Rebelo Porto; Eliana Feldberg
Journal:  Genet Mol Biol       Date:  2019-11-14       Impact factor: 1.771

  1 in total

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