Y Lv1, Y Lei2, Y Hu2, W Ding2, C Zhang3, C Fang3. 1. Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China. lv_yuefeng@126.com. 2. Department of Obstetrics, Shiyan Taihe Hospital, Hubei University of Medcine, 32 Renmin South Road, Shiyan, Hubei, China. 3. Department of Gynaecology, Shiyan Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Abstract
OBJECTIVES: The purpose of the study is to investigate the roles of miR-448 in ovarian cancer. METHODS: miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber. RESULTS: miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression. CONCLUSION: Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.
OBJECTIVES: The purpose of the study is to investigate the roles of miR-448 in ovarian cancer. METHODS:miR-448 and CXCL12 mRNA expression were examined using qRT-PCR. CXCL12 promoter activity was detected by luciferase activity system. Cell proliferation was assayed by MTT or colony formation. Migration and invasion was assayed by transwell chamber. RESULTS:miR-448 expression was usually under-expressed in ovarian cancer tissues and cell lines compared with their normal ones. Ectopic expression of miR-448 inhibited cell proliferation, migration and invasion in ovarian cancer cells. Moreover, bioinformatic prediction suggested that CXCL12 was a target gene of miR-448. We also demonstrated that restored expression of CXCL12 dampened miR-448-mediated suppression of tumor progression, which suggests the important role of miR-448 in tumor progression. CONCLUSION: Our data indicate that miR-448 functions as a tumor suppressor in ovarian cancer, which exerts its activity by suppressing the expression of CXCL12.
Authors: Sanjeev K Srivastava; Aamir Ahmad; Haseeb Zubair; Orlandric Miree; Seema Singh; Rodney P Rocconi; Jennifer Scalici; Ajay P Singh Journal: Cancer Lett Date: 2017-05-24 Impact factor: 8.679
Authors: Tomasz Powrózek; Paweł Krawczyk; Dariusz M Kowalski; Barbara Kuźnar-Kamińska; Kinga Winiarczyk; Marta Olszyna-Serementa; Halina Batura-Gabryel; Janusz Milanowski Journal: Tumour Biol Date: 2015-09-04