Sandra Fabrico de Melo1, Henrique Ravanhol Frigeri2, Izabella Castilhos Ribeiro dos Santos-Weiss1, Rosângela Roginski Réa3, Emanuel Maltempi de Souza4, Dayane Alberton1, Fabiane Gomes de Moraes Rego1, Geraldo Picheth5. 1. Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil. 2. Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil; Health and Biosciences School, Pontifical Catholic University of Parana, Curitiba, Parana, Brazil. 3. Endocrinology and Metabolism Service (SEMPR), Clinical Hospital, Federal University of Parana, Brazil. 4. Department of Biochemistry and Molecular Biology, Federal University of Parana, Brazil. 5. Post-Graduate Program in Pharmaceutical Science, Federal University of Parana, Brazil. Electronic address: gpicheth@ufpr.br.
Abstract
OBJECTIVE: To investigate the association between fat mass and obesity-associated (FTO) gene polymorphisms rs8050136C>A and rs9939609T>A, and transcription factor 7-like 2 (TCF7L2) gene polymorphisms rs12255372G>T and rs7903146C>T, in a sample group of pregnant Euro-Brazilian women with or without gestational diabetes mellitus (GDM). METHODS: Subjects were classified as either healthy pregnant control (n=200) or GDM (n=200) according to the 2010 criteria of the American Diabetes Association. The polymorphisms were genotyped using fluorescent probes (TaqMan®). RESULTS: All groups were in the Hardy-Weinberg equilibrium. The genotype and allele frequencies of the examined polymorphisms did not exhibit significant difference (P>0.05) between the groups. In the healthy and GDM pregnant women groups, the A-allele frequencies (95% CI) of FTO polymorphisms rs8050136 and rs9939609 were 39% (34-44%); 38% (33-43%) and 40% (35-45%); 41% (36-46%), respectively; and the T-allele frequencies of TCF7L2 polymorphisms rs12255372 and rs7903146 were 30% (26-35%), 32% (27-37%) and 29% (25-34%), 36% (31-41%), respectively. CONCLUSION: The examined polymorphisms were not associated with GDM in the Euro-Brazilian population studied.
OBJECTIVE: To investigate the association between fat mass and obesity-associated (FTO) gene polymorphisms rs8050136C>A and rs9939609T>A, and transcription factor 7-like 2 (TCF7L2) gene polymorphisms rs12255372G>T and rs7903146C>T, in a sample group of pregnant Euro-Brazilian women with or without gestational diabetes mellitus (GDM). METHODS: Subjects were classified as either healthy pregnant control (n=200) or GDM (n=200) according to the 2010 criteria of the American Diabetes Association. The polymorphisms were genotyped using fluorescent probes (TaqMan®). RESULTS: All groups were in the Hardy-Weinberg equilibrium. The genotype and allele frequencies of the examined polymorphisms did not exhibit significant difference (P>0.05) between the groups. In the healthy and GDM pregnant women groups, the A-allele frequencies (95% CI) of FTO polymorphisms rs8050136 and rs9939609 were 39% (34-44%); 38% (33-43%) and 40% (35-45%); 41% (36-46%), respectively; and the T-allele frequencies of TCF7L2 polymorphisms rs12255372 and rs7903146 were 30% (26-35%), 32% (27-37%) and 29% (25-34%), 36% (31-41%), respectively. CONCLUSION: The examined polymorphisms were not associated with GDM in the Euro-Brazilian population studied.
Authors: Ana Barabash; Johanna D Valerio; Nuria Garcia de la Torre; Inés Jimenez; Laura Del Valle; Verónica Melero; Carla Assaf-Balut; Manuel Fuentes; Elena Bordiu; Alejandra Durán; Miguel A Herraiz; Nuria Izquierdo; María J Torrejón; Paz de Miguel; Isabelle Runkle; Miguel A Rubio; Alfonso L Calle-Pascual Journal: Metabol Open Date: 2020-11-26
Authors: B Ortega-Contreras; A Armella; J Appel; D Mennickent; J Araya; M González; E Castro; A M Obregón; L Lamperti; J Gutiérrez; E Guzmán-Gutiérrez Journal: Front Physiol Date: 2022-04-04 Impact factor: 4.755