Irena Kolouchová1, Karel Sigler2, Olga Schreiberová3, Jan Masák3, Tomáš Řezanka4. 1. Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: irena.kolouchova@vscht.cz. 2. Institute of Microbiology, CAS, Vídeňská 1083, 142 20 Prague, Czech Republic. 3. Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. 4. Department of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic; Institute of Microbiology, CAS, Vídeňská 1083, 142 20 Prague, Czech Republic.
Abstract
Palmitoleic acid is found in certain dairy products and has broad applications in medicine and cosmetics. We tried to find a suitable producer of this acid among traditional biotechnological yeast species (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) characterized by high biomass yield and Candida krusei, Yarrowia lipolytica and Trichosporon cutaneum accumulating large amounts of lipids. The main factor affecting the content of palmitoleic acid was found to be the C/N ratio in the culture medium, with ammonium sulfate as an optimum nitrogen source leading to highest biomass yield with concomitantly increased lipid accumulation, and an increased content of ω6-linoleic acid, the precursor of prostaglandins, leukotrienes, and thromboxanes. We found that C. krusei can be conveniently used for the purpose, albeit only under certain cultivation conditions, whereas S. cerevisiae can produce high and stable amounts of palmitoleic acid in a broad range of cultivation conditions ranging from conventional to nutrient limitations.
Palmitoleic acid is found in certain dairy products and has broad applications in medicine and cosmetics. We tried to find a suitable producer of this acid among traditional biotechnological n class="Species">yeast species (Kluyveromyces polysporus, Torulaspora delbrueckii, Saccharomyces cerevisiae) characterized by high biomass yield and Candida krusei, Yarrowia lipolytica and Trichosporon cutaneum accumulating large amounts of lipids. The main factor affecting the content of palmitoleic acid was found to be the C/N ratio in the culture medium, with ammonium sulfate as an optimum nitrogen source leading to highest biomass yield with concomitantly increased lipid accumulation, and an increased content of ω6-linoleic acid, the precursor of prostaglandins, leukotrienes, and thromboxanes. We found that C. krusei can be conveniently used for the purpose, albeit only under certain cultivation conditions, whereas S. cerevisiae can produce high and stable amounts of palmitoleic acid in a broad range of cultivation conditions ranging from conventional to nutrient limitations.
Authors: Irena Kolouchova; Karel Sigler; Michal Zimola; Tomas Rezanka; Olga Matatkova; Jan Masak Journal: World J Microbiol Biotechnol Date: 2016-06-23 Impact factor: 3.312
Authors: Dennis Lamers; Nick van Biezen; Dirk Martens; Linda Peters; Eric van de Zilver; Nicole Jacobs-van Dreumel; René H Wijffels; Christien Lokman Journal: BMC Biotechnol Date: 2016-05-27 Impact factor: 2.563
Authors: Olga Matatkova; Lucia Gharwalova; Michal Zimola; Tomas Rezanka; Jan Masak; Irena Kolouchova Journal: Int J Anal Chem Date: 2017-10-10 Impact factor: 1.885