Ying Chen1,2, Xiaofu Zhuo2, Yisheng Lin3, Wenhua Huang2, Jingrong Xiao1, Jia Zeng2, Li Jiang2, Cen Chen2, Haijuan Lin2, Markus Dettke4. 1. Fujian Provincial Cancer Hospital, the Affiliated Hospital of Fujian Medical University. 2. Fujian Provincial Blood Center, Fuzhou, China. 3. Quanzhou Blood Center, Quanzhou, China. 4. Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria.
Abstract
BACKGROUND: Recent genome-wide association studies in Caucasians suggested that an association exists between the ABO gene locus and soluble levels of P-selectin (sP-selectin). However, it is unclear if the relationship corresponds to the phenotypic expression of ABO groups or is present in different ethnic groups. The aim of this study was to verify this observation at both genotypic and phenotypic levels in a healthy Chinese population. STUDY DESIGN AND METHODS: The ABO blood groups were determined by both phenotypes and genotypes in 440 healthy Chinese Han volunteers, while P-selectin levels were evaluated for sP-selectin and total platelet P-selectin (pP-selectin). RESULTS: ABO phenotyping and quantitative analysis of individual sP-selectin plasma levels were combined to demonstrate that individuals phenotypically expressing the A antigen have approximately 20% lower sP-selectin plasma levels than those carrying the B or O phenotype (p < 0.0001), but that no difference exists between A and AB and between B and O phenotypes. Genotyping data revealed that the presence of the A gene could be attributed to the observed difference in phenotype comparison, with no difference between A/A, A/B, and A/O genotypes. There were also no associations between ABO blood groups, either phenotypes or genotypes, and pP-selectin levels. CONCLUSION: This study demonstrated an association between sP-selectin levels and ABO groups in a Chinese Han population, implicating its generalizability to other ethnic groups. This finding will improve the understanding of the mechanism of ABO blood group-associated diseases.
BACKGROUND: Recent genome-wide association studies in Caucasians suggested that an association exists between the ABO gene locus and soluble levels of P-selectin (sP-selectin). However, it is unclear if the relationship corresponds to the phenotypic expression of ABO groups or is present in different ethnic groups. The aim of this study was to verify this observation at both genotypic and phenotypic levels in a healthy Chinese population. STUDY DESIGN AND METHODS: The ABO blood groups were determined by both phenotypes and genotypes in 440 healthy Chinese Han volunteers, while P-selectin levels were evaluated for sP-selectin and total platelet P-selectin (pP-selectin). RESULTS:ABO phenotyping and quantitative analysis of individual sP-selectin plasma levels were combined to demonstrate that individuals phenotypically expressing the A antigen have approximately 20% lower sP-selectin plasma levels than those carrying the B or O phenotype (p < 0.0001), but that no difference exists between A and AB and between B and O phenotypes. Genotyping data revealed that the presence of the A gene could be attributed to the observed difference in phenotype comparison, with no difference between A/A, A/B, and A/O genotypes. There were also no associations between ABO blood groups, either phenotypes or genotypes, and pP-selectin levels. CONCLUSION: This study demonstrated an association between sP-selectin levels and ABO groups in a Chinese Han population, implicating its generalizability to other ethnic groups. This finding will improve the understanding of the mechanism of ABO blood group-associated diseases.
Authors: Nicholas B Larson; Elizabeth J Bell; Paul A Decker; Mindy Pike; Christina L Wassel; Michael Y Tsai; James S Pankow; Weihong Tang; Naomi Q Hanson; Kristine Alexander; Neil A Zakai; Mary Cushman; Suzette J Bielinski Journal: Atherosclerosis Date: 2016-06-07 Impact factor: 5.162
Authors: Nicholas B Larson; Paul A Decker; Christina L Wassel; James S Pankow; Weihong Tang; Naomi Q Hanson; Michael Y Tsai; Suzette J Bielinski Journal: Hum Genet Date: 2016-02-16 Impact factor: 4.132