Hui Cheng1, Yi-fang Kuang2, Yang Liu3, Yi Wang4, Zheng-hao Xu4, Feng Gao2, Shi-hong Zhang4, Mei-ping Ding2, Zhong Chen5. 1. 1] Department of Neurology, Epilepsy Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China [2] Department of Neurology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China. 2. Department of Neurology, Epilepsy Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China. 3. Department of Neurology, Zhejiang Hospital, Hangzhou 310058, China. 4. Department of Pharmacology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. 5. 1] Department of Neurology, Epilepsy Center, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China [2] Department of Pharmacology, Key Laboratory of Medical Neurobiology of the Ministry of Health of China, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Abstract
AIM: To investigate the anti-epileptic effects of deep brain stimulation targeting the external globus palladium (GPe) in rats. METHODS: For inducing amygdala kindling and deep brain stimulation, bipolar stainless-steel electrodes were implanted in SD rats into right basolateral amygdala and right GPe, respectively. The effects of deep brain stimulation were evaluated in the amygdala kindling model, maximal electroshock model (MES) and pentylenetetrazole (PTZ) model. Moreover, the background EEGs in the amygdala and GPe were recorded. RESULTS: Low-frequency stimulation (0.1 ms, 1 Hz, 15 min) at the GPe slowed the progression of seizure stages and shortened the after-discharge duration (ADD) during kindling acquisition. Furthermore, low-frequency stimulation significantly decreased the incidence of generalized seizures, suppressed the average stage, and shortened the cumulative ADD and generalized seizure duration in fully kindled rats. In addition, low-frequency stimulation significantly suppressed the average stage of MES-induced seizures and increased the latency to generalized seizures in the PTZ model. High-frequency stimulation (0.1 ms, 130 Hz, 5 min) at the GPe had no anti-epileptic effect and even aggravated epileptogenesis induced by amygdala kindling. EEG analysis showed that low-frequency stimulation at the GPe reversed the increase in delta power, whereas high-frequency stimulation at the GPe had no such effect. CONCLUSION: Low-frequency stimulation, but not high-frequency stimulation, at the GPe exerts therapeutic effect on temporal lobe epilepsy and tonic-colonic generalized seizures, which may be due to interference with delta rhythms. The results suggest that modulation of GPe activity using low-frequency stimulation or drugs may be a promising epilepsy treatment.
AIM: To investigate the anti-epileptic effects of deep brain stimulation targeting the external globus palladium (GPe) in rats. METHODS: For inducing amygdala kindling and deep brain stimulation, bipolar stainless-steel electrodes were implanted in SD rats into right basolateral amygdala and right GPe, respectively. The effects of deep brain stimulation were evaluated in the amygdala kindling model, maximal electroshock model (MES) and pentylenetetrazole (PTZ) model. Moreover, the background EEGs in the amygdala and GPe were recorded. RESULTS: Low-frequency stimulation (0.1 ms, 1 Hz, 15 min) at the GPe slowed the progression of seizure stages and shortened the after-discharge duration (ADD) during kindling acquisition. Furthermore, low-frequency stimulation significantly decreased the incidence of generalized seizures, suppressed the average stage, and shortened the cumulative ADD and generalized seizure duration in fully kindled rats. In addition, low-frequency stimulation significantly suppressed the average stage of MES-induced seizures and increased the latency to generalized seizures in the PTZ model. High-frequency stimulation (0.1 ms, 130 Hz, 5 min) at the GPe had no anti-epileptic effect and even aggravated epileptogenesis induced by amygdala kindling. EEG analysis showed that low-frequency stimulation at the GPe reversed the increase in delta power, whereas high-frequency stimulation at the GPe had no such effect. CONCLUSION: Low-frequency stimulation, but not high-frequency stimulation, at the GPe exerts therapeutic effect on temporal lobe epilepsy and tonic-colonic generalized seizures, which may be due to interference with delta rhythms. The results suggest that modulation of GPe activity using low-frequency stimulation or drugs may be a promising epilepsy treatment.
Authors: Robert Fisher; Vicenta Salanova; Thomas Witt; Robert Worth; Thomas Henry; Robert Gross; Kalarickal Oommen; Ivan Osorio; Jules Nazzaro; Douglas Labar; Michael Kaplitt; Michael Sperling; Evan Sandok; John Neal; Adrian Handforth; John Stern; Antonio DeSalles; Steve Chung; Andrew Shetter; Donna Bergen; Roy Bakay; Jaimie Henderson; Jacqueline French; Gordon Baltuch; William Rosenfeld; Andrew Youkilis; William Marks; Paul Garcia; Nicolas Barbaro; Nathan Fountain; Carl Bazil; Robert Goodman; Guy McKhann; K Babu Krishnamurthy; Steven Papavassiliou; Charles Epstein; John Pollard; Lisa Tonder; Joan Grebin; Robert Coffey; Nina Graves Journal: Epilepsia Date: 2010-03-17 Impact factor: 5.864
Authors: Ivan Rektor; Jan Tomčík; Michal Mikl; Radek Mareček; Milan Brázdil; Irena Rektorová Journal: Brain Topogr Date: 2013-02-12 Impact factor: 3.020