Jun feng Su1, Sheng Chen Zheng2, Ting lin Huang2, Fang Ma3, Si Cheng Shao2, Shao Fei Yang2, Li na Zhang2. 1. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. 2. School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. 3. State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: mafang@hit.edu.cn.
Abstract
Acinetobacter sp. SZ28 exhibited efficient autotrophic denitrification ability using Mn(2+) as an electron donor. Sequence amplification identified the presence of the nirS gene. Meteorological chromatography analysis showed that N2 was produced as an end product. Response surface methodology experiments showed that the maximum removal of nitrate occurred under the following conditions: Mn(2+) concentration of 143.56 mg/L, C/N ratio of 6.82, initial pH of 5.17, and temperature of 34.26 °C, where the initial Mn(2+) concentration produced the largest effect. In the groundwater experiment, nitrate levels decreased from 1.63 mg/L to 0 mg/L. Three-dimensional fluorescence analysis showed a decrease in the peak intensity of the original humus. Humus and the small-molecule amino acid tryptophan were detected. These results demonstrated that strain SZ28 is a suitable candidate for the simultaneous removal of nitrogen and Mn(2+) in groundwater treatment.
Acinetobacter sp. SZ28 exhibited efficient autotrophic denitrification ability using n class="Chemical">Mn(2+) as an electron donor. Sequence amplification identified the presence of the nirS gene. Meteorological chromatography analysis showed that N2 was produced as an end product. Response surface methodology experiments showed that the maximum removal of nitrate occurred under the following conditions: Mn(2+) concentration of 143.56 mg/L, C/N ratio of 6.82, initial pH of 5.17, and temperature of 34.26 °C, where the initial Mn(2+) concentration produced the largest effect. In the groundwater experiment, nitrate levels decreased from 1.63 mg/L to 0 mg/L. Three-dimensional fluorescence analysis showed a decrease in the peak intensity of the original humus. Humus and the small-molecule amino acid tryptophan were detected. These results demonstrated that strain SZ28 is a suitable candidate for the simultaneous removal of nitrogen and Mn(2+) in groundwater treatment.
Authors: Lin Cai; Ren-Mao Tian; Guowei Zhou; Haoya Tong; Yue Him Wong; Weipeng Zhang; Apple Pui Yi Chui; James Y Xie; Jian-Wen Qiu; Put O Ang; Sheng Liu; Hui Huang; Pei-Yuan Qian Journal: Sci Rep Date: 2018-02-05 Impact factor: 4.379