Chul Ho Jang1, Yong Beom Cho2, Yoon Seok Jang3, Min Seong Kim4, Geun Hyung Kim5. 1. Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea. Electronic address: chulsavio@hanmail.net. 2. Department of Otolaryngology, Chonnam National University Medical School, Gwangju, South Korea. 3. Ecobio Laboratory, Gwangju, South Korea. 4. Department of Bio-Mechatronics, Sungkyunkwan University (SKKU), Suwon, South Korea. 5. Department of Bio-Mechatronics, Sungkyunkwan University (SKKU), Suwon, South Korea. Electronic address: gkimbme@skku.edu.
Abstract
OBJECTIVE: In this study, we developed a biocompatible and biodegradable vancomycin (VM)-eluting electrospun poly(ɛ-caprolactone) (PCL)/polyethylene oxide (PEO)/VM/PCL nanofiber mat. The purpose of this study was to evaluate the efficacy of this nanofiber mat for preventing methicillin-resistant Staphylococcus aureus (MRSA) biofilm on the ossicular prostheses and otitis media. MATERIALS AND METHODS: Fabrication of VM eluting nanofiber mat was carried out using PCL and PEO. The release of VM was assessed by spectrophotometry in vitro. The antibacterial effect was evaluated by inhibition zone test and biofilm formation test in vitro. And prevention of MRSA infection by guinea pig was assessed by otoendoscopy, temporal bone CT and histopathologic examination in vivo. RESULTS: VM released followed a triphasic pattern. VM eluting nanofiber mat prevented MRSA biofilm formation on the surface of ossicular prostheses regardless of materials in vitro, and MRSA otitis media in vivo. Otoendoscopy, temporal bone CT, and histopathologic findings revealed well ventilated middle ear. CONCLUSION: From these results, VM eluting nanofiber mat may be a promising method for prevention of MRSA periprosthetic infection and biofilm formation.
OBJECTIVE: In this study, we developed a biocompatible and biodegradable vancomycin (VM)-eluting electrospun poly(ɛ-caprolactone) (PCL)/polyethylene oxide (PEO)/VM/PCL nanofiber mat. The purpose of this study was to evaluate the efficacy of this nanofiber mat for preventing methicillin-resistant Staphylococcus aureus (MRSA) biofilm on the ossicular prostheses and otitis media. MATERIALS AND METHODS: Fabrication of VM eluting nanofiber mat was carried out using PCL and PEO. The release of VM was assessed by spectrophotometry in vitro. The antibacterial effect was evaluated by inhibition zone test and biofilm formation test in vitro. And prevention of MRSA infection by guinea pig was assessed by otoendoscopy, temporal bone CT and histopathologic examination in vivo. RESULTS: VM released followed a triphasic pattern. VM eluting nanofiber mat prevented MRSA biofilm formation on the surface of ossicular prostheses regardless of materials in vitro, and MRSA otitis media in vivo. Otoendoscopy, temporal bone CT, and histopathologic findings revealed well ventilated middle ear. CONCLUSION: From these results, VM eluting nanofiber mat may be a promising method for prevention of MRSA periprosthetic infection and biofilm formation.
Authors: Maria Magana; Christina Sereti; Anastasios Ioannidis; Courtney A Mitchell; Anthony R Ball; Emmanouil Magiorkinis; Stylianos Chatzipanagiotou; Michael R Hamblin; Maria Hadjifrangiskou; George P Tegos Journal: Clin Microbiol Rev Date: 2018-04-04 Impact factor: 26.132
Authors: Jakub Spałek; Przemysław Ociepa; Piotr Deptuła; Ewelina Piktel; Tamara Daniluk; Grzegorz Król; Stanisław Góźdź; Robert Bucki; Sławomir Okła Journal: Int J Mol Sci Date: 2022-02-25 Impact factor: 5.923