Chunfeng Yun1, Jing Chen1, Chun Yang1, Yajie Li1, Jianhua Piao1, Xiaoguang Yang2. 1. National Institute of Nutrition and Health, Chinese Center for Disease Control and Prevention, Nanwei Road No. 29, Xicheng District, Beijing postcode 100050, China. 2. National Institute of Nutrition and Health, Chinese Center for Disease Control and Prevention, Nanwei Road No. 29, Xicheng District, Beijing postcode 100050, China. Electronic address: xgyangcdc@vip.sina.com.
Abstract
BACKGROUND: We compared the utility of two 25-OH-D immunoassays to a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for assessing human serum samples from the Chinese population. METHODS: In total, 451 Chinese serum samples were tested using the IDS 25-Hydroxy Vitamin D EIA kits and the LC-MS/MS 25-OH-D method. Of these, 239 samples were also analysed using DiaSorin 25-Hydroxyvitamin D RIA kits. RESULTS: The regression coefficients (r) between LC-MS/MS and immunoassays were 0.8430 (95% CI: 0.8140-0.8678) for IDS-EIA and 0.8187 (95% CI: 0.7720-0.8566) for DiaSorin-RIA. Using the Passing-Bablok analysis, IDS-EIA and DiaSorin-RIA yielded results equivalent to those from LC-MS/MS. However, IDS-EIA showed negative bias and DiaSorin-RIA showed positive bias when compared to LC-MS/MS, as indicated by the Bland-Altman plots. By the kappa test, both IDS-EIA and DiaSorin-RIA were in acceptable agreement with LC-MS/MS in the assessment of vitamin D deficiency and insufficiency. CONCLUSION: Although some systematic bias was found, the DiaSorin-RIA and IDS-EIA kits met the minimum performance goal for 25-OH-D measurement and can be recommended for routine use in the Chinese population. However, further studies are needed to assess 25-OH-D measurement immunoassays with lower bias and that are more suitable for the Chinese population.
BACKGROUND: We compared the utility of two 25-OH-D immunoassays to a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for assessing human serum samples from the Chinese population. METHODS: In total, 451 Chinese serum samples were tested using the IDS25-Hydroxy Vitamin D EIA kits and the LC-MS/MS 25-OH-D method. Of these, 239 samples were also analysed using DiaSorin 25-Hydroxyvitamin D RIA kits. RESULTS: The regression coefficients (r) between LC-MS/MS and immunoassays were 0.8430 (95% CI: 0.8140-0.8678) for IDS-EIA and 0.8187 (95% CI: 0.7720-0.8566) for DiaSorin-RIA. Using the Passing-Bablok analysis, IDS-EIA and DiaSorin-RIA yielded results equivalent to those from LC-MS/MS. However, IDS-EIA showed negative bias and DiaSorin-RIA showed positive bias when compared to LC-MS/MS, as indicated by the Bland-Altman plots. By the kappa test, both IDS-EIA and DiaSorin-RIA were in acceptable agreement with LC-MS/MS in the assessment of vitamin Ddeficiency and insufficiency. CONCLUSION: Although some systematic bias was found, the DiaSorin-RIA and IDS-EIA kits met the minimum performance goal for 25-OH-D measurement and can be recommended for routine use in the Chinese population. However, further studies are needed to assess 25-OH-D measurement immunoassays with lower bias and that are more suitable for the Chinese population.
Authors: Kristi R Van Winden; Allison Bearden; Naoko Kono; Toni Frederick; Eva Operskalski; Alice Stek; Raj Pandian; Lorayne Barton; Andrea Kovacs Journal: Am J Perinatol Date: 2019-07-31 Impact factor: 3.079
Authors: Allison Bearden; Kristi Van Winden; Toni Frederick; Naoko Kono; Eva Operskalski; Raj Pandian; Lorayne Barton; Alice Stek; Andrea Kovacs Journal: PLoS One Date: 2020-02-13 Impact factor: 3.752