Ashraf A Elghzaly1, Shereen S Metwally2, Farha A El-Chennawi3, Maha A Elgayaar4, Youssef M Mosaad5, Ehab E El-Toraby6, Mohsen M Hegab7, Saleh M Ibrahim8. 1. Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: drashrafantar@mans.edu.eg. 2. Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: shereen_salah@ymail.com. 3. Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: dr_farha@mans.edu.eg. 4. Dermatology and Andrology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: elgayar_m@yahoo.com. 5. Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: youssefmosaad@yahoo.com. 6. Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: Ehabeltoraby@yahoo.com. 7. Molecular Medicine Group, Department of Reproductive Medicine, Division of Medical Research, National Research Center, Egypt. Electronic address: mohsenhegab@gmail.com. 8. Dermatology Department, Lübeck University, Lübeck, Germany. Electronic address: Saleh.Ibrahim@uksh.de.
Abstract
OBJECTIVE: To replicate a single nucleotide polymorphism (SNP) of known genes for lupus (IRF5 rs10488631, PTPN22 rs2476601, BLK rs2736340 and TNFAIP3 rs5029939) and other autoimmune diseases (CD28 rs1980422, IL2RA rs2104286 and KIF5A rs1678542) on a newly studied Egyptian cohort to investigate the genetic disparity with different studied ethnic groups in relation to lupus susceptibility. METHODS: 170 Egyptian patients from Egypt Delta with SLE and 241 matched healthy controls were genotyped by Taqman real time PCR for the selected SNPs. RESULTS: The results revealed significant association with IRF5 (p<0.0001) and PTPN22 (p=0.008) and insignificant association with KIF5A, CD28, IL2RA, BLK and TNFAIP3 genes. CONCLUSIONS: This study may provide an additional evidence for the association between IRF5 and PTPN22 and lupus susceptibility and may exclude it for CD28, IL2RA, and KIF5A.
OBJECTIVE: To replicate a single nucleotide polymorphism (SNP) of known genes for lupus (IRF5rs10488631, PTPN22rs2476601, BLKrs2736340 and TNFAIP3rs5029939) and other autoimmune diseases (CD28rs1980422, IL2RArs2104286 and KIF5Ars1678542) on a newly studied Egyptian cohort to investigate the genetic disparity with different studied ethnic groups in relation to lupus susceptibility. METHODS: 170 Egyptian patients from Egypt Delta with SLE and 241 matched healthy controls were genotyped by Taqman real time PCR for the selected SNPs. RESULTS: The results revealed significant association with IRF5 (p<0.0001) and PTPN22 (p=0.008) and insignificant association with KIF5A, CD28, IL2RA, BLK and TNFAIP3 genes. CONCLUSIONS: This study may provide an additional evidence for the association between IRF5 and PTPN22 and lupus susceptibility and may exclude it for CD28, IL2RA, and KIF5A.
Authors: Olanrewaju B Morenikeji; Jessica L Metelski; Megan E Hawkes; Anna L Capria; Brooke N Seamans; Catherine O Falade; Olusola Ojurongbe; Bolaji N Thomas Journal: Microorganisms Date: 2020-01-23