Liyan Jiang1, Yan Liu2, Guodong Yan2, Yuxiao Cui2, Qiyue Cheng2, Zaixiao Zhang2, Qingfan Meng2, Lirong Teng2, Xiaodong Ren3,4. 1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130023, China. 2. School of Life Science, Jilin University, Changchun, 130023, China. 3. School of Life Science, Jilin University, Changchun, 130023, China. renxiaodong@jlu.edu.cn. 4. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130023, China. renxiaodong@jlu.edu.cn.
Abstract
OBJECTIVES: To optimize nisin production in Lactococcus lactis by using different aeration and fermentation strategies. RESULTS: The nisin titer and specific nisin production rate reached maximum values of 11,900 IU/ml and 4110 IU/g/h, respectively, in aerobic batch fermentation with glucose as C source. These values were higher than in anaerobic batch fermentation (10,700 IU/ml and 3260 IU/g/h, respectively). The maximum specific nisin production rates appeared earlier in aerobic batch fermentation, which suggests that nisin production is stimulated by aeration. Different fermentation strategies were compared: maximum nisin production (15,400 IU/ml) was achieved with fed-batch fermentation with a variable rate of feeding under aerobic conditions. CONCLUSION: Nisin production can be stimulated by aeration, which goes against the typical conditions involving strict anaerobiosis.
OBJECTIVES: To optimize nisin production in Lactococcus lactis by using different aeration and fermentation strategies. RESULTS: The nisin titer and specific nisin production rate reached maximum values of 11,900 IU/ml and 4110 IU/g/h, respectively, in aerobic batch fermentation with glucose as C source. These values were higher than in anaerobic batch fermentation (10,700 IU/ml and 3260 IU/g/h, respectively). The maximum specific nisin production rates appeared earlier in aerobic batch fermentation, which suggests that nisin production is stimulated by aeration. Different fermentation strategies were compared: maximum nisin production (15,400 IU/ml) was achieved with fed-batch fermentation with a variable rate of feeding under aerobic conditions. CONCLUSION: Nisin production can be stimulated by aeration, which goes against the typical conditions involving strict anaerobiosis.
Authors: Elvis L Ongey; Lara Santolin; Saskia Waldburger; Lorenz Adrian; Sebastian L Riedel; Peter Neubauer Journal: Front Microbiol Date: 2019-09-13 Impact factor: 5.640