Stela Vujosevic1, Alessandra Micera2, Silvia Bini1, Marianna Berton1, Graziana Esposito2, Edoardo Midena3. 1. Department of Ophthalmology University of Padova, Padova, Italy. 2. Fondazione G. B. Bietti, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy. 3. Department of Ophthalmology University of Padova, Padova, Italy 2Fondazione G. B. Bietti, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy.
Abstract
PURPOSE: To identify early biomarkers of retinal Müller cell activation in diabetic eyes with or without clinically detectable signs of diabetic retinopathy (DR). METHODS: This study was a cross-sectional comparative case series. The aqueous humor (AH) of 34 eyes was collected in 12 healthy controls, 11 diabetic patients without DR, and 11 diabetic patients with nonproliferative DR. Full ophthalmic examination and spectral-domain optical coherence tomography were performed in all eyes. Glial fibrillary acidic protein (GFAP), aquaporin 1 (AQP1), and aquaporin 4 (AQP4) were quantified in AH samples as biomarkers of Müller cell activity by ELISA. Statistical analysis was performed with ANOVA followed by Tukey-Kramer post hoc test. RESULTS: There was no significant difference in the age among the three groups. Mean concentration of GFAP, AQP1, and AQP4 significantly increased in diabetic eyes versus controls (P < 0.05, for each comparison). Glial fibrillary acidic protein and AQP1 showed an approximate 2-fold increase, whereas AQP4 showed an approximate 25-fold increase in diabetics with DR versus controls. In diabetics without DR, AQP4 showed an approximate 6-fold increase versus controls. CONCLUSIONS: Glial fibrillary acidic protein, AQP1, and AQP4-biomarkers of Müller cell activity-are significantly increased in human eyes with diabetes, confirming that Müller cells are precociously affected by diabetes mellitus.
PURPOSE: To identify early biomarkers of retinal Müller cell activation in diabetic eyes with or without clinically detectable signs of diabetic retinopathy (DR). METHODS: This study was a cross-sectional comparative case series. The aqueous humor (AH) of 34 eyes was collected in 12 healthy controls, 11 diabeticpatients without DR, and 11 diabeticpatients with nonproliferative DR. Full ophthalmic examination and spectral-domain optical coherence tomography were performed in all eyes. Glial fibrillary acidic protein (GFAP), aquaporin 1 (AQP1), and aquaporin 4 (AQP4) were quantified in AH samples as biomarkers of Müller cell activity by ELISA. Statistical analysis was performed with ANOVA followed by Tukey-Kramer post hoc test. RESULTS: There was no significant difference in the age among the three groups. Mean concentration of GFAP, AQP1, and AQP4 significantly increased in diabetic eyes versus controls (P < 0.05, for each comparison). Glial fibrillary acidic protein and AQP1 showed an approximate 2-fold increase, whereas AQP4 showed an approximate 25-fold increase in diabetics with DR versus controls. In diabetics without DR, AQP4 showed an approximate 6-fold increase versus controls. CONCLUSIONS:Glial fibrillary acidic protein, AQP1, and AQP4-biomarkers of Müller cell activity-are significantly increased in human eyes with diabetes, confirming that Müller cells are precociously affected by diabetes mellitus.
Authors: Sadie K Dierschke; Allyson L Toro; William P Miller; Siddharth Sunilkumar; Michael D Dennis Journal: J Biol Chem Date: 2020-05-31 Impact factor: 5.157
Authors: Mikael Klingeborn; W Michael Dismuke; Catherine Bowes Rickman; W Daniel Stamer Journal: Prog Retin Eye Res Date: 2017-04-29 Impact factor: 21.198