Mariano Licciardi1,2,3, Anna Li Volsi4, Carla Sardo4, Nicolò Mauro4, Gennara Cavallaro4, Gaetano Giammona4,5. 1. Department of Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Palermo, Italy. mariano.licciardi@unipa.it. 2. Mediterranean Center for Human Health Advanced Biotechnologies (Med-CHAB), Palermo, Italy. mariano.licciardi@unipa.it. 3. Laboratory of Biocompatible Polymers, Department of Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Via Archirafi, 32 90123, Palermo, Italy. mariano.licciardi@unipa.it. 4. Department of Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Palermo, Italy. 5. Mediterranean Center for Human Health Advanced Biotechnologies (Med-CHAB), Palermo, Italy.
Abstract
PURPOSE: An inulin based polycation (Inu-EDA) has been synthesized by the grafting of ethylenediamine molecules onto inulin backbone. The obtained inulin copolymer has been though to coat SPIONs (IC-SPIONs) and obtain stable magnetoplexes by complexation of IC-SPIONs with a model duplexed siRNA, for improving oligonucleotide transfection efficiency. METHODS: The physical-chemical characteristics of IC-SPIONs and IC-SPIONs/siRNA magnetoplexes have been investigated by scanning and transmission electron microscopies, dynamic light scattering, FT-IR and qualitative surface elementary analysis. Cell compatibility and internalization in vitro of IC-SPIONs have been evaluated by MTS and fluorescence microscopy respectively on cancer (HCT116) and normal human (16HBE) cells. The efficiency of gene silencing effect of magnetoplexes was studied on both tumoral (JHH6) and non tumoral (16HBE) cell lines also by applying an external magnet. RESULTS: IC-SPIONs showed dimension of 30 nm and resulted cytocompatible on the tested cell lines; in the presence of an external magnet, the magnetic force enhanced the IC-SPIONs uptake inside cells. Magnetically improved transfection was observed in 16HBE cells under magnetofective conditions, in accordance with the IC-SPIONs uptake enhancement in the presence of an external magnet. CONCLUSIONS: These findings support the potential application of this system as a magnetically targeted drug delivery system. Graphical Abstract Magnetically improved siRNA transfection in cells under magnetofective conditions upon uptake enhancement of IC-SPIONs in the presence of an external magnet.
PURPOSE: An inulin based polycation (Inu-EDA) has been synthesized by the grafting of ethylenediamine molecules onto inulin backbone. The obtained inulin copolymer has been though to coat SPIONs (IC-SPIONs) and obtain stable magnetoplexes by complexation of IC-SPIONs with a model duplexed siRNA, for improving oligonucleotide transfection efficiency. METHODS: The physical-chemical characteristics of IC-SPIONs and IC-SPIONs/siRNA magnetoplexes have been investigated by scanning and transmission electron microscopies, dynamic light scattering, FT-IR and qualitative surface elementary analysis. Cell compatibility and internalization in vitro of IC-SPIONs have been evaluated by MTS and fluorescence microscopy respectively on cancer (HCT116) and normal human (16HBE) cells. The efficiency of gene silencing effect of magnetoplexes was studied on both tumoral (JHH6) and non tumoral (16HBE) cell lines also by applying an external magnet. RESULTS: IC-SPIONs showed dimension of 30 nm and resulted cytocompatible on the tested cell lines; in the presence of an external magnet, the magnetic force enhanced the IC-SPIONs uptake inside cells. Magnetically improved transfection was observed in 16HBE cells under magnetofective conditions, in accordance with the IC-SPIONs uptake enhancement in the presence of an external magnet. CONCLUSIONS: These findings support the potential application of this system as a magnetically targeted drug delivery system. Graphical Abstract Magnetically improved siRNA transfection in cells under magnetofective conditions upon uptake enhancement of IC-SPIONs in the presence of an external magnet.
Authors: C Sardo; R Farra; M Licciardi; B Dapas; C Scialabba; G Giammona; M Grassi; G Grassi; G Cavallaro Journal: Eur J Pharm Sci Date: 2015-04-04 Impact factor: 4.384
Authors: Sara Prijic; Lara Prosen; Maja Cemazar; Janez Scancar; Rok Romih; Jaka Lavrencak; Vladimir B Bregar; Andrej Coer; Mojca Krzan; Andrej Znidarsic; Gregor Sersa Journal: Biomaterials Date: 2012-03-18 Impact factor: 12.479
Authors: Yan Deng; Chi Chiu Wang; Kwong Wai Choy; Quan Du; Jiao Chen; Qin Wang; Lu Li; Tony Kwok Hung Chung; Tao Tang Journal: Gene Date: 2014-01-07 Impact factor: 3.688
Authors: Anna Li Volsi; Dorleta Jimenez de Aberasturi; Malou Henriksen-Lacey; Gaetano Giammona; Mariano Licciardi; Luis M Liz-Marzán Journal: J Mater Chem B Date: 2016-01-04 Impact factor: 6.331
Authors: Franklin Afinjuomo; Paris Fouladian; Thomas G Barclay; Yunmei Song; Nikolai Petrovsky; Sanjay Garg Journal: Polymers (Basel) Date: 2020-05-01 Impact factor: 4.329