Omer Faruk Karatas1,2, Betul Yuceturk1,3, Ilknur Suer1, Mehmet Yilmaz4, Harun Cansiz4, Mustafa Solak5, Michael Ittmann6, Mustafa Ozen1,6,7. 1. Department of Medical Genetics, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 2. Molecular Biology and Genetics Department, Erzurum Technical University, Erzurum, Turkey. 3. Advanced Genomics and Bioinformatics Research Center, The Scientific and Technological Research Council of Turkey (TUBITAK), Gebze, Kocaeli, Turkey. 4. Department of Otorhinolaryngology, Cerrahpasa Medical School, Istanbul University, Istanbul, Turkey. 5. Department of Medical Genetics, Afyon Kocatepe University, Afyon, Turkey. 6. Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas. 7. Department of Molecular Biology and Genetics, Biruni University, Topkapi, Istanbul, Turkey.
Abstract
BACKGROUND: Laryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. Incidences of laryngeal SCC have been reported to increase recently. In this study, we aimed to explore the biological effects of miR-145 on laryngeal cancer cells. METHODS: The relative miR-145 level in laryngeal SCC tumor tissues and normal samples was investigated. Then, Hep-2 cells were utilized for functional analysis of miR-145. The proliferation abilities of transfected cells were measured using MTS assay. Scratch assay and single colony migration assay were performed to observe the alterations in migration behavior of transfected cells. Caspase assay and cell cycle analysis were used to investigate the underlying reasons of proliferative inhibition in cells in which miR-145 is overexpressed. Moreover, expression of SOX2 was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis in Hep-2 cells upon miR-145 transfection and its expression was evaluated in tumor and normal tissue sample of the larynx. RESULTS: The miR-145 expression in laryngeal SCC tumor samples has been shown to be downregulated. The miR-145 overexpression caused inhibition of proliferation and migration in Hep-2 cells through induction of apoptosis and cell cycle arrest. The SOX2 level was demonstrated to be overexpressed in tumor samples and its expression was significantly decreased in miR-145 overexpressed Hep-2 cells. CONCLUSION: We have demonstrated the deregulation of miR-145 and SOX2 in laryngeal SCC. Based on these results, we propose that miR-145, as an important regulator of SOX2, carries crucial roles in laryngeal SCC tumorigenesis.
BACKGROUND: Laryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. Incidences of laryngeal SCC have been reported to increase recently. In this study, we aimed to explore the biological effects of miR-145 on laryngeal cancer cells. METHODS: The relative miR-145 level in laryngeal SCC tumor tissues and normal samples was investigated. Then, Hep-2 cells were utilized for functional analysis of miR-145. The proliferation abilities of transfected cells were measured using MTS assay. Scratch assay and single colony migration assay were performed to observe the alterations in migration behavior of transfected cells. Caspase assay and cell cycle analysis were used to investigate the underlying reasons of proliferative inhibition in cells in which miR-145 is overexpressed. Moreover, expression of SOX2 was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis in Hep-2 cells upon miR-145 transfection and its expression was evaluated in tumor and normal tissue sample of the larynx. RESULTS: The miR-145 expression in laryngeal SCC tumor samples has been shown to be downregulated. The miR-145 overexpression caused inhibition of proliferation and migration in Hep-2 cells through induction of apoptosis and cell cycle arrest. The SOX2 level was demonstrated to be overexpressed in tumor samples and its expression was significantly decreased in miR-145 overexpressed Hep-2 cells. CONCLUSION: We have demonstrated the deregulation of miR-145 and SOX2 in laryngeal SCC. Based on these results, we propose that miR-145, as an important regulator of SOX2, carries crucial roles in laryngeal SCC tumorigenesis.
Authors: Omer Faruk Karatas; Ilknur Suer; Betul Yuceturk; Mehmet Yilmaz; Yusif Hajiyev; Chad J Creighton; Michael Ittmann; Mustafa Ozen Journal: Tumour Biol Date: 2015-10-22
Authors: K Bednarek; K Kiwerska; M Szaumkessel; M Bodnar; M Kostrzewska-Poczekaj; A Marszalek; J Janiszewska; A Bartochowska; J Jackowska; M Wierzbicka; R Grenman; K Szyfter; M Giefing; M Jarmuz-Szymczak Journal: Tumour Biol Date: 2016-02-24
Authors: Guilherme Rabinowits; Michaela Bowden; Ludmila M Flores; Sigitas Verselis; Victoria Vergara; Vickie Y Jo; Nicole Chau; Jochen Lorch; Peter S Hammerman; Tom Thomas; Laura A Goguen; Donald Annino; Jonathan D Schoenfeld; Danielle N Margalit; Roy B Tishler; Robert I Haddad Journal: Front Oncol Date: 2017-08-29 Impact factor: 6.244