| Literature DB >> 26083493 |
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Abstract
[This corrects the article DOI: 10.1371/journal.pone.0122922.].Entities:
Year: 2015 PMID: 26083493 PMCID: PMC4470582 DOI: 10.1371/journal.pone.0130506
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Nonspecific LAMP reaction test using ctrA primer sets.
| First designed | Second designed | Nm LAMP | |
|---|---|---|---|
| Reaction temperature | 65°C | 67°C | 67°C |
| Trial no. 1 | 58.1 min | - | - |
| 2 | 87.4 | - | - |
| 3 | - | 49.2 | - |
| 4 | 78.4 | - | - |
| 5 | - | - | - |
| 6 | - | - | - |
| 7 | - | - | - |
aAmplification reaction using the first designed ctrA primer set.
bAmplification reaction using the second designed ctrA primer set.
cAmplification reaction using the final ctrA primer set with a mutation in FIP (Nm LAMP).
dA sample of each trial used distilled water.
eDetection time determined by a Loopamp real-time turbidimeter.
f-, no false positive reaction.