Gyula Pekar1, Hanna Davies2, Agnes P Lukacs1, Lars Forsberg2, Dan Hellberg3, Jan Dumanski2, Tibor Tot1. 1. Department of Pathology and Clinical Cytology, Central Hospital Falun, Falun, Sweden. 2. Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden. 3. Centre for Clinical Research Dalarna, Uppsala University, Falun, Sweden.
Abstract
AIMS: To determine the volume of tumoral and normal breast tissue containing sufficient DNA (>2 μg/sample) for genetic platforms and biobanking, with a focus on multifocality, tumoral heterogeneity, and factors that critically influence sample acceptability. METHODS AND RESULTS: We examined 57 breast surgical specimens with multifocal (46/57) and unifocal (11/57) cancers. Punch biopsies were obtained from tissue slices under multimodal radiological guidance, and the colour-coded sampling sites were identified in large-format histology slides. The study comprised 415 DNA isolations from tumour (n = 105) and normal (n = 283) tissue, including skin (n = 27) samples. A single 2-mm core from invasive tumour contained sufficient DNA in 91.4% (96/105) of cases, depending on tumour type (3.8-108.2 μg/sample), number and size of additional foci in multifocal cases (P = 0.001), tumour consistency, and degree of necrosis. Three biopsies obtained with a 4-mm device were required from normal breast tissue, at least 10 mm from the tumour. Cold ischaemia for up to 82 min did not influence the yield of DNA. CONCLUSIONS: Radiological disease mapping is useful for guiding optimal specimen slicing and for targeting breast lesions. A single 2-mm core from tumour and multiple 4-mm cores from normal breast tissue yield adequate DNA in the majority of samples.
AIMS: To determine the volume of tumoral and normal breast tissue containing sufficient DNA (>2 μg/sample) for genetic platforms and biobanking, with a focus on multifocality, tumoral heterogeneity, and factors that critically influence sample acceptability. METHODS AND RESULTS: We examined 57 breast surgical specimens with multifocal (46/57) and unifocal (11/57) cancers. Punch biopsies were obtained from tissue slices under multimodal radiological guidance, and the colour-coded sampling sites were identified in large-format histology slides. The study comprised 415 DNA isolations from tumour (n = 105) and normal (n = 283) tissue, including skin (n = 27) samples. A single 2-mm core from invasive tumour contained sufficient DNA in 91.4% (96/105) of cases, depending on tumour type (3.8-108.2 μg/sample), number and size of additional foci in multifocal cases (P = 0.001), tumour consistency, and degree of necrosis. Three biopsies obtained with a 4-mm device were required from normal breast tissue, at least 10 mm from the tumour. Cold ischaemia for up to 82 min did not influence the yield of DNA. CONCLUSIONS: Radiological disease mapping is useful for guiding optimal specimen slicing and for targeting breast lesions. A single 2-mm core from tumour and multiple 4-mm cores from normal breast tissue yield adequate DNA in the majority of samples.
Authors: Lars A Forsberg; Chiara Rasi; Gyula Pekar; Hanna Davies; Arkadiusz Piotrowski; Devin Absher; Hamid Reza Razzaghian; Aleksandra Ambicka; Krzysztof Halaszka; Marcin Przewoźnik; Anna Kruczak; Geeta Mandava; Saichand Pasupulati; Julia Hacker; K Reddy Prakash; Ravi Chandra Dasari; Joey Lau; Nelly Penagos-Tafurt; Helena M Olofsson; Gunilla Hallberg; Piotr Skotnicki; Jerzy Mituś; Jaroslaw Skokowski; Michal Jankowski; Ewa Śrutek; Wojciech Zegarski; Eva Tiensuu Janson; Janusz Ryś; Tibor Tot; Jan P Dumanski Journal: Genome Res Date: 2015-10 Impact factor: 9.043
Authors: Natalia Filipowicz; Kinga Drężek; Monika Horbacz; Agata Wojdak; Jakub Szymanowski; Edyta Rychlicka-Buniowska; Ulana Juhas; Katarzyna Duzowska; Tomasz Nowikiewicz; Wiktoria Stańkowska; Katarzyna Chojnowska; Maria Andreou; Urszula Ławrynowicz; Magdalena Wójcik; Hanna Davies; Ewa Śrutek; Michał Bieńkowski; Katarzyna Milian-Ciesielska; Marek Zdrenka; Aleksandra Ambicka; Marcin Przewoźnik; Agnieszka Harazin-Lechowska; Agnieszka Adamczyk; Jacek Kowalski; Dariusz Bała; Dorian Wiśniewski; Karol Tkaczyński; Krzysztof Kamecki; Marta Drzewiecka; Paweł Wroński; Jerzy Siekiera; Izabela Ratnicka; Jerzy Jankau; Karol Wierzba; Jarosław Skokowski; Karol Połom; Mikołaj Przydacz; Łukasz Bełch; Piotr Chłosta; Marcin Matuszewski; Krzysztof Okoń; Olga Rostkowska; Andrzej Hellmann; Karol Sasim; Piotr Remiszewski; Marek Sierżęga; Stanisław Hać; Jarosław Kobiela; Łukasz Kaska; Michał Jankowski; Diana Hodorowicz-Zaniewska; Janusz Jaszczyński; Wojciech Zegarski; Wojciech Makarewicz; Rafał Pęksa; Joanna Szpor; Janusz Ryś; Łukasz Szylberg; Arkadiusz Piotrowski; Jan P Dumanski Journal: PLoS One Date: 2022-04-07 Impact factor: 3.240