Pierluigi Tos1, Alessandro Crosio1, Igor Pellegatta2, Luigi Valdatta2, Davide Pascal3, Stefano Geuna3, Mario Cherubino2. 1. Reconstructive Microsurgery Unit, Traumatology Department, CTO Hospital, Via Zuretti 29, 10100 Turin, Italy. 2. Plastic Surgery Unit, Department of Biotechnologies and Sciences of Life, University of Insubria/Varese, Varese, Italy. 3. Human Anatomy Laboratory, Clinical and Biological Sciences Department, University of Turin, AOU San Luigi Gonzaga, Orbassano, Italy.
Abstract
INTRODUCTION: Perineural scar formation is responsible for pain and loss of function after surgical procedures. Neurolysis and application of anti-adhesion gels are required to restore a gliding surface. We tested a carboxymethylcellulose (CMC) and polyethylene oxide (PEO) gel on mouse sciatic nerve to describe its safety and efficacy. METHODS: Adult mice underwent a surgical procedure in which we burned the muscular bed of the sciatic nerve bilaterally (Burned group) and applied anti-adhesion gel to 1 of the nerves (Burned+gel group). After 3 weeks, we studied scar tissue by biomechanical and histological evaluation. RESULTS: Both histological and biomechanical analysis showed that the gel reduced perineural scarring. The difference between the Burned and Burned+gel groups was statistically significant. CONCLUSIONS: CMC-PEO gel can reduce perineural scar tissue. In histological section, scar tissue was present in both groups, but in the Burned+gel group a gliding surface was identified between scar and nerve.
INTRODUCTION: Perineural scar formation is responsible for pain and loss of function after surgical procedures. Neurolysis and application of anti-adhesion gels are required to restore a gliding surface. We tested a carboxymethylcellulose (CMC) and polyethylene oxide (PEO) gel on mouse sciatic nerve to describe its safety and efficacy. METHODS: Adult mice underwent a surgical procedure in which we burned the muscular bed of the sciatic nerve bilaterally (Burned group) and applied anti-adhesion gel to 1 of the nerves (Burned+gel group). After 3 weeks, we studied scar tissue by biomechanical and histological evaluation. RESULTS: Both histological and biomechanical analysis showed that the gel reduced perineural scarring. The difference between the Burned and Burned+gel groups was statistically significant. CONCLUSIONS:CMC-PEO gel can reduce perineural scar tissue. In histological section, scar tissue was present in both groups, but in the Burned+gel group a gliding surface was identified between scar and nerve.
Authors: Mario Cherubino; Igor Pellegatta; Alessandro Crosio; Luigi Valdatta; Stefano Geuna; Rosalba Gornati; Pierluigi Tos Journal: PLoS One Date: 2017-04-26 Impact factor: 3.240
Authors: Hailey P Huddleston; Neil V Shah; Joey S Kurtzman; Qurratul-Ain Dar; Hanbin Wang; John Carter; Westley T Hayes; Alba Avoricani; Karan Dua; Steven M Koehler Journal: Plast Reconstr Surg Glob Open Date: 2021-11-11