Literature DB >> 2608157

Intermediate filament proteins immunologically related to desmin in astrocytes: a study of chicken spinal cord by two-dimensional gel electrophoresis and immunoblotting.

D Dahl1, C J Crosby, A Bignami.   

Abstract

Co-migration experiments by two-dimensional SDS-PAGE using chicken spinal cord extracts and desmin purified from chicken gizzard showed that desmin is not present in spinal cord. However, by the immunoblotting procedure, desmin antibodies recognized 3 spinal cord antigens with different molecular weights and isoelectric points than desmin and the glial fibrillary acidic (GFA) protein. These antigens which also reacted with GFA protein antibodies were not identified in chicken gizzard extracts. The reactivity of the antigens with a monoclonal antibody recognizing an epitope common to most intermediate filament proteins (1) suggests that immunostaining of astrocytes with desmin antibodies (2, 3) is due to the presence of new intermediate filament proteins immunologically related to desmin.

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Year:  1989        PMID： 2608157     DOI： 10.1007/bf00965936

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  21 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (Pt K2) by immunofluorescence microscopy.

Authors:  M Osborn; W W Franke; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

3.  Heterogeneity of desmin, the muscle-type intermediate filament protein, in blood vessels and astrocytes.

Authors:  D Dahl; S Zapatka; A Bignami
Journal:  Histochemistry       Date:  1986

Review 4.  Intermediate filaments in nervous tissue.

Authors:  D Dahl; A Bignami
Journal:  Cell Muscle Motil       Date:  1985

5.  Molecular interactions in intermediate-sized filaments revealed by chemical cross-linking. Heteropolymers of vimentin and glial filament protein in cultured human glioma cells.

Authors:  R A Quinlan; W W Franke
Journal:  Eur J Biochem       Date:  1983-05-16

6.  Immunohistological localization of desmin, the muscle-type 100 A filament protein, in rat astrocytes and Müller glia.

Authors:  D Dahl; A Bignami
Journal:  J Histochem Cytochem       Date:  1982-03       Impact factor: 2.479

7.  Study of glial fibrillary acidic protein in a human glioma cell line grown in culture and as a solid tumor.

Authors:  J W Bigbee; D D Bigner; C Pegram; L F Eng
Journal:  J Neurochem       Date:  1983-02       Impact factor: 5.372

8.  Immunohistochemical differences between neurofilaments in perikarya, dendrites and axons. Immunofluorescence study with antisera raised to neurofilament polypeptides (200K, 150K, 70K) isolated by anion exchange chromatography.

Authors:  D Dahl
Journal:  Exp Cell Res       Date:  1983-12       Impact factor: 3.905

9.  A new protein (J1-31 antigen, 30 kD) is expressed by astrocytes, Müller glia and ependyma.

Authors:  R Predy; D Singh; R Bhatnagar; R Singh; S K Malhotra
Journal:  Biosci Rep       Date:  1987-06       Impact factor: 3.840

10.  Enhanced expression of a protein antigen (J1-31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord.

Authors:  R Predy; S K Malhotra; G D Das
Journal:  J Neurosci Res       Date:  1988-04       Impact factor: 4.164
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  1 in total

1.  Rosenthal fibers producing a granular cell appearance in a glioblastoma.

Authors:  M R Del Bigio; J H Deck
Journal:  Acta Neuropathol       Date:  1993       Impact factor: 17.088
  1 in total

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