Lübo Tian1, Xiaoxiao Chen1, Liming He1, Xuejun Fan2, Ying Shi1, Guolong Gao1, Yu Yang1. 1. Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China. 2. Sichuan Entry-exit Inspection and Quarantine Bureau International Travel Healthcare Center, Chengdu 610041, China; Email: h121260@126.com.
Abstract
OBJECTIVE: To monitor the the genotype and subgenotype c of hepatitis C among the immigrations. To identify the new genotype and trace the transmission. METHODS: From 2009 to 2013, 235 positive Anti-HCV samples were collected from Sichuan, Liaoning, Guangdong, Shenzhen and Yunnan ITHC. Specific PCR primers were used to amplify the HCV Core, then the PCR products were sequenced by genetic analyzer. The genotypes were identified by aligning the GenBank reference sequences and constructing the phylogenetic tree of Core. RESULTS: One hundred and twenty-nine samples showed HCV-RNA positive (54.9%) in 235 samples which were anti-HCV positive. We detected six kinds of genotypes in 115 cases of sequncing successfully, of which 72 cases of genotype 1 (G1, 62.6%), followed by G3 (18.2%), G2 (9.6%), G4 (6%), G6 (2.6%) and G5 (0.9%). Genotype 1b was the most common subtype, accounting for 47% of all infections. The phylogenetic tress indicated the HCV 4 strains in immigrations had high affinity with that in Egypt and Europe, while HCV 6 strains closed with that in China. CONCLUSIONS: HCV 1b is the advantage of popular genotype in HCV carriers. Subtype 4 may be a possible new genotype transmited into China. The immigrations may be the sources of new genotype of HCV.
OBJECTIVE: To monitor the the genotype and subgenotype c of hepatitis C among the immigrations. To identify the new genotype and trace the transmission. METHODS: From 2009 to 2013, 235 positive Anti-HCV samples were collected from Sichuan, Liaoning, Guangdong, Shenzhen and Yunnan ITHC. Specific PCR primers were used to amplify the HCV Core, then the PCR products were sequenced by genetic analyzer. The genotypes were identified by aligning the GenBank reference sequences and constructing the phylogenetic tree of Core. RESULTS: One hundred and twenty-nine samples showed HCV-RNA positive (54.9%) in 235 samples which were anti-HCV positive. We detected six kinds of genotypes in 115 cases of sequncing successfully, of which 72 cases of genotype 1 (G1, 62.6%), followed by G3 (18.2%), G2 (9.6%), G4 (6%), G6 (2.6%) and G5 (0.9%). Genotype 1b was the most common subtype, accounting for 47% of all infections. The phylogenetic tress indicated the HCV 4 strains in immigrations had high affinity with that in Egypt and Europe, while HCV 6 strains closed with that in China. CONCLUSIONS: HCV 1b is the advantage of popular genotype in HCV carriers. Subtype 4 may be a possible new genotype transmited into China. The immigrations may be the sources of new genotype of HCV.
Authors: X M Li; R X Qiu; C H Song; Q H Huang; X D Wang; Z T Hu; X Z He; X Y Ye; X G Huang; F F Zheng; G X Lin Journal: Epidemiol Infect Date: 2017-10-30 Impact factor: 4.434