Literature DB >> 26080699

Delivery of siRNA to ovarian cancer cells using laser-activated carbon nanoparticles.

Aritra Sengupta1, Roman Mezencev2, John F McDonald2, Mark R Prausnitz1.   

Abstract

AIM: The RNAi-mediated knockdown of gene expression is an attractive tool for research and therapeutic purposes but its implementation is challenging. Here we report on a new method based on photoacoustic delivery of siRNA developed to address some of these challenges. MATERIALS &
METHODS: Physical properties and photoacoustic emission of carbon black (CB) particles upon near-infrared laser irradiation were characterized. Next, ovarian cancer cells Hey A8-F8 were exposed to near-infrared nanosecond laser pulses in the presence of siRNA targeting EGFR gene and CB particles. The intracellular delivery of siRNA and silencing of the target gene were determined by specific qPCR assays. RESULTS &
CONCLUSION: Laser-activated CB nanoparticles generated photoacoustic emission and enabled intracellular delivery of siRNA and significant knockdown of its target EGFR mRNA. This physical method represents a new promising approach to targeted therapeutic delivery of siRNA.

Entities:  

Keywords:  EGFR; intracellular delivery; nanosecond laser; photoacoustics; siRNA

Mesh：

Substances：

Year:  2015        PMID： 26080699      PMCID： PMC4491916          DOI： 10.2217/nnm.15.27

Source DB:  PubMed          Journal:  Nanomedicine (Lond)        ISSN： 1743-5889            Impact factor:   5.307


  45 in total

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5.  Inhibition of HPV 16 E6 oncogene expression by RNA interference in vitro and in vivo.

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6.  Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivo.

Authors:  Fumitaka Takeshita; Yoshiko Minakuchi; Shunji Nagahara; Kimi Honma; Hideo Sasaki; Kotaro Hirai; Takumi Teratani; Nachi Namatame; Yusuke Yamamoto; Koji Hanai; Takashi Kato; Akihiko Sano; Takahiro Ochiya
Journal:  Proc Natl Acad Sci U S A       Date:  2005-08-09       Impact factor: 11.205

7.  Evidence of RNAi in humans from systemically administered siRNA via targeted nanoparticles.

Authors:  Mark E Davis; Jonathan E Zuckerman; Chung Hang J Choi; David Seligson; Anthony Tolcher; Christopher A Alabi; Yun Yen; Jeremy D Heidel; Antoni Ribas
Journal:  Nature       Date:  2010-03-21       Impact factor: 49.962

8.  Delivery of molecules into cells using carbon nanoparticles activated by femtosecond laser pulses.

Authors:  Prerona Chakravarty; Wei Qian; Mostafa A El-Sayed; Mark R Prausnitz
Journal:  Nat Nanotechnol       Date:  2010-07-18       Impact factor: 39.213

9.  Dynamics of photoinduced endosomal release of polyplexes.

Authors:  K G de Bruin; C Fella; M Ogris; E Wagner; N Ruthardt; C Bräuchle
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10.  The first targeted delivery of siRNA in humans via a self-assembling, cyclodextrin polymer-based nanoparticle: from concept to clinic.

Authors:  Mark E Davis
Journal:  Mol Pharm       Date:  2009 May-Jun       Impact factor: 4.939
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  6 in total

1.  Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels.

Authors:  Cong Truc Huynh; Minh Khanh Nguyen; Mantas Naris; Gulen Yesilbag Tonga; Vincent M Rotello; Eben Alsberg
Journal:  Nanomedicine (Lond)       Date:  2016-06-01       Impact factor: 5.307

2.  Energy Transfer Mechanisms during Molecular Delivery to Cells by Laser-Activated Carbon Nanoparticles.

Authors:  Aritra Sengupta; Michael D Gray; Sean C Kelly; Stefany Y Holguin; Naresh N Thadhani; Mark R Prausnitz
Journal:  Biophys J       Date:  2017-03-28       Impact factor: 4.033

3.  Serum Protects Cells and Increases Intracellular Delivery of Molecules by Nanoparticle-Mediated Photoporation.

Authors:  Simple Kumar; Eunice Lazau; Carter Kim; Naresh N Thadhani; Mark R Prausnitz
Journal:  Int J Nanomedicine       Date:  2021-05-31

4.  Photoresponsive miR-34a/Nanoshell Conjugates Enable Light-Triggered Gene Regulation to Impair the Function of Triple-Negative Breast Cancer Cells.

Authors:  Megan N Dang; Carolina Gomez Casas; Emily S Day
Journal:  Nano Lett       Date:  2020-12-11       Impact factor: 11.189

Review 5.  The Application of Carbon Nanomaterials in Sensing, Imaging, Drug Delivery and Therapy for Gynecologic Cancers: An Overview.

Authors:  Changji Xiao; Changming Li; Jun Hu; Lirong Zhu
Journal:  Molecules       Date:  2022-07-13       Impact factor: 4.927

Review 6.  The Application of Inorganic Nanoparticles in Molecular Targeted Cancer Therapy: EGFR Targeting.

Authors:  Meng Sun; Ting Wang; Leijiao Li; Xiangyang Li; Yutong Zhai; Jiantao Zhang; Wenliang Li
Journal:  Front Pharmacol       Date:  2021-07-12       Impact factor: 5.810
  6 in total

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