Yuliana Monsalve1, Giovanni Tosi2, Barbara Ruozi2, Daniela Belletti2, Antonietta Vilella3, Michele Zoli3, Maria Angela Vandelli2, Flavio Forni2, Betty L López1, Ligia Sierra1. 1. Grupo de Investigación Ciencia de los Materiales, Instituto de Química, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Calle 70 N° 52-21, Medellín, Colombia. 2. Pharmaceutical Technology, Te.Far.T.I. group, Department of Life Sciences, University of Modena & Reggio Emilia, Via Campi 183,41124 Modena, Italy. 3. Department of Biomedical, Metabolic & Neural Sciences, University of Modena & Reggio Emilia, Via Campi 213, 41124 Modena, Italy.
Abstract
AIM: Drug targeting to the CNS is challenging due to the presence of blood-brain barrier. We investigated chitosan (Cs) nanoparticles (NPs) as drug transporter system across the blood-brain barrier, based on mAb OX26 modified Cs. MATERIALS & METHODS: Cs NPs functionalized with PEG, modified and unmodified with OX26 (Cs-PEG-OX26) were prepared and chemico-physically characterized. These NPs were administered (intraperitoneal) in mice to define their ability to reach the brain. RESULTS: Brain uptake of OX26-conjugated NPs is much higher than of unmodified NPs, because: long-circulating abilities (conferred by PEG), interaction between cationic Cs and brain endothelium negative charges and OX26 TfR receptor affinity. CONCLUSION: Cs-PEG-OX26 NPs are promising drug delivery system to the CNS.
AIM: Drug targeting to the CNS is challenging due to the presence of blood-brain barrier. We investigated chitosan (Cs) nanoparticles (NPs) as drug transporter system across the blood-brain barrier, based on mAb OX26 modified Cs. MATERIALS & METHODS:Cs NPs functionalized with PEG, modified and unmodified with OX26 (Cs-PEG-OX26) were prepared and chemico-physically characterized. These NPs were administered (intraperitoneal) in mice to define their ability to reach the brain. RESULTS: Brain uptake of OX26-conjugated NPs is much higher than of unmodified NPs, because: long-circulating abilities (conferred by PEG), interaction between cationic Cs and brain endothelium negative charges and OX26 TfR receptor affinity. CONCLUSION:Cs-PEG-OX26 NPs are promising drug delivery system to the CNS.
Entities:
Keywords:
blood–brain barrier; chitosan; drug targeting; monoclonal antibody OX26; nanoparticles