Ximin Wang1, Ming Zhang1, Yujiao Zhang1, Xinxing Xie1, Weizong Wang1, Zhan Li1, Mei Gao1, Zhongsu Wang1, Yinglong Hou2. 1. From the School of Medicine (X.W., Y.Z., W.W., Z.L.), Departments of Cardiology (X.X., M.G., Z.W., Y.H.), and Intensive Care Unit (M.Z.), Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China. 2. From the School of Medicine (X.W., Y.Z., W.W., Z.L.), Departments of Cardiology (X.X., M.G., Z.W., Y.H.), and Intensive Care Unit (M.Z.), Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China. houyinglong2010@hotmail.com.
Abstract
BACKGROUND: The long-term effects of ganglionated plexi ablation on electrophysiological characteristics and neuron remodeling in target atrial tissues remain unclear. METHODS AND RESULTS: Dogs in group 1 (control, n=8) were not subjected to ganglionated plexi ablation and observed for 1 month, and dogs in groups 2 to 4 (ablation groups, n=8 each) underwent ablation of the right-sided ganglionated plexi and observed for 1, 6, and 12 months, respectively. Atrial electrophysiological characteristics were examined before ablation, immediately and continuously after ablation. Target atrial tissues were subjected to immunohistochemical staining and Western blot analysis. Atrial effective refractory period was significantly prolonged immediately after ablation (P<0.001), and persisted for 1 month (P<0.05). Nerve densities decreased 1 month after ablation (P<0.001). These parameters reverted to preablation levels after 6 and 12 months. In the ablation groups, atrial fibrillation was induced in 5 of 8 dogs after 1 month and in all animals after 6 and 12 months. Atrial fibrillation was not observed in the control group and in the experimental groups immediately after ablation. Moreover, the expression of the growth-associated protein 43 was upregulated after ablation. CONCLUSIONS: Ganglionated plexi ablation effectively prolonged atrial effective refractory period for a short period, but the long-term effects on atrial effective refractory period and the suppression of atrial fibrillation induction were not persistent. Targeted atrial neuron remodeling may be an important mechanism underlying the observed electrophysiological changes.
BACKGROUND: The long-term effects of ganglionated plexi ablation on electrophysiological characteristics and neuron remodeling in target atrial tissues remain unclear. METHODS AND RESULTS:Dogs in group 1 (control, n=8) were not subjected to ganglionated plexi ablation and observed for 1 month, and dogs in groups 2 to 4 (ablation groups, n=8 each) underwent ablation of the right-sided ganglionated plexi and observed for 1, 6, and 12 months, respectively. Atrial electrophysiological characteristics were examined before ablation, immediately and continuously after ablation. Target atrial tissues were subjected to immunohistochemical staining and Western blot analysis. Atrial effective refractory period was significantly prolonged immediately after ablation (P<0.001), and persisted for 1 month (P<0.05). Nerve densities decreased 1 month after ablation (P<0.001). These parameters reverted to preablation levels after 6 and 12 months. In the ablation groups, atrial fibrillation was induced in 5 of 8 dogs after 1 month and in all animals after 6 and 12 months. Atrial fibrillation was not observed in the control group and in the experimental groups immediately after ablation. Moreover, the expression of the growth-associated protein 43 was upregulated after ablation. CONCLUSIONS: Ganglionated plexi ablation effectively prolonged atrial effective refractory period for a short period, but the long-term effects on atrial effective refractory period and the suppression of atrial fibrillation induction were not persistent. Targeted atrial neuron remodeling may be an important mechanism underlying the observed electrophysiological changes.
Authors: Alvise Del Monte; Luigi Pannone; Antonio Bisignani; Thiago G Osório; Saverio Iacopino; Gian-Battista Chierchia; Carlo de Asmundis Journal: Front Cardiovasc Med Date: 2022-07-28
Authors: Martin van Zyl; Mariam Khabsa; Jason A Tri; Thomas P Ladas; Omar Z Yasin; Adetola O Ladejobi; John Reilly; Barry O'Brien; Kenneth Coffey; Samuel J Asirvatham Journal: J Innov Card Rhythm Manag Date: 2022-07-15