Literature DB >> 2607785

Calibration of 125I-polymer standards with 125I-brain paste standards for use in quantitative receptor autoradiography.

A J Nazarali1, J S Gutkind, J M Saavedra.   

Abstract

125I-Polymer standards were calibrated by interpolation of their optical densities in [125I]-brain paste standard curves to obtain dpm/mg protein. There was a linear relationship between the calibrated polymer standards and the dpm/mg polymer, as provided by the manufacturer. One dpm/mg polymer was equivalent to 7.34 +/- 0.22 dpm/mg protein. Receptor quantification in selected rat brain areas with comparison to either brain paste or calibrated polymer standards yielded similar results.

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Year:  1989        PMID： 2607785     DOI： 10.1016/0165-0270(89)90135-0

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  24 in total

Review 1.  Angiotensin and cerebral blood flow.

Authors:  J M Saavedra; Y Nishimura
Journal:  Cell Mol Neurobiol       Date:  1999-10       Impact factor: 5.046

2.  Effect of NMDA-induced lesion of the subfornical organ on the angiotensin II binding sites density and acetylcholinesterase or NADPH-diphorase activities in the lamina terminalis of the rat brain.

Authors:  S S Guilhaume; F M Corrêa
Journal:  Cell Mol Neurobiol       Date:  2001-02       Impact factor: 5.046

3.  Influence of maternal melatonin on melatonin receptors in rat offspring.

Authors:  M Zitouni; M Masson-Pévet; F Gauer; P Pévet
Journal:  J Neural Transm Gen Sect       Date:  1995

4.  An attempt to correlate brain areas containing melatonin-binding sites with rhythmic functions: a study in five hibernator species.

Authors:  M Masson-Pévet; D George; A Kalsbeek; M Saboureau; N Lakhdar-Ghazal; P Pévet
Journal:  Cell Tissue Res       Date:  1994-10       Impact factor: 5.249

5.  Type-1 angiotensin receptors are expressed and transported in motor and sensory axons of rat sciatic nerves.

Authors:  Hui Tang; Jaroslav Pavel; Juan M Saavedra; Stephen Brimijoin
Journal:  Neuropeptides       Date:  2009-02-23       Impact factor: 3.286

6.  Kinin B1 receptor enhances the oxidative stress in a rat model of insulin resistance: outcome in hypertension, allodynia and metabolic complications.

Authors:  Jenny Pena Dias; Sébastien Talbot; Jacques Sénécal; Pierre Carayon; Réjean Couture
Journal:  PLoS One       Date:  2010-09-07       Impact factor: 3.240

7.  Key role for spinal dorsal horn microglial kinin B1 receptor in early diabetic pain neuropathy.

Authors:  Sébastien Talbot; Emna Chahmi; Jenny Pena Dias; Réjean Couture
Journal:  J Neuroinflammation       Date:  2010-06-29       Impact factor: 8.322

8.  Balloon angioplasty enhances the expression of angiotensin II AT1 receptors in neointima of rat aorta.

Authors:  M Viswanathan; C Strömberg; A Seltzer; J M Saavedra
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808

9.  Increased angiotensin II AT1 receptor mRNA and binding in spleen and lung of AT2 receptor gene disrupted mice.

Authors:  Jaroslav Pavel; José A Terrón; Julius Benicky; Alicia Falcón-Neri; Amita Rachakonda; Tadashi Inagami; Juan M Saavedra
Journal:  Regul Pept       Date:  2009-09-17

10.  Cellular localization of kinin B1 receptor in the spinal cord of streptozotocin-diabetic rats with a fluorescent [Nalpha-Bodipy]-des-Arg9-bradykinin.

Authors:  Sébastien Talbot; Patrick Théberge-Turmel; Dalinda Liazoghli; Jacques Sénécal; Pierrette Gaudreau; Réjean Couture
Journal:  J Neuroinflammation       Date:  2009-03-26       Impact factor: 8.322
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