Honggen Liu1, Yong Liu2, Fanming Kong1, Wen Xin1, Xiaojiang Li1, Han Liang3, Yingjie Jia1. 1. Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China. 2. Department of Gastric Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China. 3. Department of Gastric Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China. Electronic address: tjlianghanzl@126.com.
Abstract
BACKGROUND: The aim of this study was to investigate the messenger RNA and protein expressions of SET and MYND domain-containing protein 3 (SMYD3) and transforming growth factor-β1 (TGF-β1) in gastric cancer (GC) and to explore the correlations between these proteins and the biologic behavior of GC. STUDY DESIGN: Expressions of SMYD3 and TGF-β1 were detected by real-time quantitative reverse transcription polymerase chain reaction and Western blot in GC tissues and adjacent nontumor tissues. In addition, SMYD3 and TGF-β1 expressions were analyzed by immunohistochemistry in formalin-fixed samples from 166 GC patients. RESULTS: The messenger RNA and protein expression levels of SMYD3 and TGF-β1 in GC tissues were significantly higher than those in adjacent nontumor tissues. A significantly positive correlation was found between SMYD3 expression and TGF-β1 expression in GC tissues. In addition, the size of the primary tumor and lymph node metastasis were identified as the independently relative factors of SMYD3 expression in GC tissues, and lymph node metastasis was identified as the independently relative factor of TGF-β1 expression. Multivariate analysis demonstrated that the degree of differentiation, serosal invasion, lymph node metastasis, SMYD3 expression, and TGF-β1 expression were the independent prognostic indicators for GC. Transforming growth factor-β1 expression was one of the optimal prognostic predictors of patients identified using the Cox regression with Akaike Information Criterion value calculation. CONCLUSIONS: SET and MYND domain-containing protein 3 expression and TGF-β1 expression in GC tissues were significantly and positively correlated. High expression levels of SMYD3 and TGF-β1 can indicate poor prognoses for GC patients.
BACKGROUND: The aim of this study was to investigate the messenger RNA and protein expressions of SET and MYND domain-containing protein 3 (SMYD3) and transforming growth factor-β1 (TGF-β1) in gastric cancer (GC) and to explore the correlations between these proteins and the biologic behavior of GC. STUDY DESIGN: Expressions of SMYD3 and TGF-β1 were detected by real-time quantitative reverse transcription polymerase chain reaction and Western blot in GC tissues and adjacent nontumor tissues. In addition, SMYD3 and TGF-β1 expressions were analyzed by immunohistochemistry in formalin-fixed samples from 166 GC patients. RESULTS: The messenger RNA and protein expression levels of SMYD3 and TGF-β1 in GC tissues were significantly higher than those in adjacent nontumor tissues. A significantly positive correlation was found between SMYD3 expression and TGF-β1 expression in GC tissues. In addition, the size of the primary tumor and lymph node metastasis were identified as the independently relative factors of SMYD3 expression in GC tissues, and lymph node metastasis was identified as the independently relative factor of TGF-β1 expression. Multivariate analysis demonstrated that the degree of differentiation, serosal invasion, lymph node metastasis, SMYD3 expression, and TGF-β1 expression were the independent prognostic indicators for GC. Transforming growth factor-β1 expression was one of the optimal prognostic predictors of patients identified using the Cox regression with Akaike Information Criterion value calculation. CONCLUSIONS:SET and MYND domain-containing protein 3 expression and TGF-β1 expression in GC tissues were significantly and positively correlated. High expression levels of SMYD3 and TGF-β1 can indicate poor prognoses for GC patients.
Authors: Dai-Shi Su; Junya Qu; Mark Schulz; Chuck W Blackledge; Hongyi Yu; Jenny Zeng; Joelle Burgess; Alexander Reif; Melissa Stern; Raman Nagarajan; Melissa Baker Pappalardi; Kristen Wong; Alan P Graves; William Bonnette; Liping Wang; Patricia Elkins; Beth Knapp-Reed; Jeffrey D Carson; Charles McHugh; Helai Mohammad; Ryan Kruger; Juan Luengo; Dirk A Heerding; Caretha L Creasy Journal: ACS Med Chem Lett Date: 2019-12-27 Impact factor: 4.345
Authors: Hung-An Ting; Denise de Almeida Nagata; Andrew J Rasky; Carrie-Anne Malinczak; Ivan P Maillard; Matthew A Schaller; Nicholas W Lukacs Journal: Mucosal Immunol Date: 2018-07-23 Impact factor: 7.313