Arko Sen1,2, Nicole Heredia3, Marie-Claude Senut1, Matthew Hess3,4, Susan Land3, Wen Qu2, Kurt Hollacher5, Mary O Dereski3, Douglas M Ruden1,5. 1. Institute of Environmental Health Sciences, Wayne State University, 2727 Second Avenue, Detroit, MI 48201, USA. 2. Department of Pharmacology, Wayne State University, Room 4000, 2727 Second Avenue, Detroit, MI 48201, USA. 3. Department of Obstetrics & Gynecology, Wayne State University, 3750 Woodward Avenue, Detroit, MI 48201, USA. 4. CS Mott Center for Human Growth & Development, Wayne State University, 275 E Hancock St, Detroit, MI 48201, USA. 5. Union College, 807 Union St, Schenectady, NY 12308, USA.
Abstract
AIMS: In this paper, we tested the hypothesis that early life lead (Pb) exposure associated DNA methylation (5 mC) changes are dependent on the sex of the child and can serve as biomarkers for Pb exposure. METHODS: In this pilot study, we measured the 5mC profiles of DNA extracted from dried blood spots (DBS) in a cohort of 43 children (25 males and 18 females; ages from 3 months to 5 years) from Detroit. Result & Discussion: We found that the effect of Pb-exposure on the 5-mC profiles can be separated into three subtypes: affected methylation loci which are conserved irrespective of the sex of the child (conserved); affected methylation loci unique to males (male-specific); and affected methylation loci unique to females (female-specific).
AIMS: In this paper, we tested the hypothesis that early life lead (Pb) exposure associated DNA methylation (5 mC) changes are dependent on the sex of the child and can serve as biomarkers for Pb exposure. METHODS: In this pilot study, we measured the 5mC profiles of DNA extracted from dried blood spots (DBS) in a cohort of 43 children (25 males and 18 females; ages from 3 months to 5 years) from Detroit. Result & Discussion: We found that the effect of Pb-exposure on the 5-mC profiles can be separated into three subtypes: affected methylation loci which are conserved irrespective of the sex of the child (conserved); affected methylation loci unique to males (male-specific); and affected methylation loci unique to females (female-specific).
Entities:
Keywords:
DNA methylation; blood lead levels; differential methylation; dried blood spots; epigenetics; gene specific; lead; whole blood
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