Yan-Hui Li1, Gaigai Zhang2, Qinghua Cui3. 1. Institute of Cardiovascular Sciences, Peking University Health Science Center. 2. Department of Geriatrics and Gerontology, Beijing Huaxin Hospital, the First Affiliated Hospital of Tsinghua University and. 3. Department of Biomedical Informatics, Peking University Health Science Center, Beijing, China.
Abstract
MOTIVATION: Pseudouridine (Ψ), catalyzed by pseudouridine synthase (PUS), is the most abundant RNA modification and has important cellular functions. Developing an algorithm to identify Ψ sites is an important work. And it is better if the algorithm could assign which PUS modifies the Ψ sites. Here, we developed PPUS (http://lyh.pkmu.cn/ppus/), the first web server to predict PUS-specific Ψ sites. PPUS: employed support vector machine as the classifier and used nucleotides around Ψ sites as the features. Currently, PPUS: could accurately predict new Ψ sites for PUS1, PUS4 and PUS7 in yeast and PUS4 in human. PPUS: is well designed and friendly to user. AVAILABILITY AND IMPLEMENTATION: Our web server is available freely for non-commercial purposes at: http://lyh.pkmu.cn/ppus/ CONTACT: liyanhui@bjmu.edu.cn or cuiqinghua@hsc.pku.edu.cn.
MOTIVATION:Pseudouridine (Ψ), catalyzed by pseudouridine synthase (PUS), is the most abundant RNA modification and has important cellular functions. Developing an algorithm to identify Ψ sites is an important work. And it is better if the algorithm could assign which PUS modifies the Ψ sites. Here, we developed PPUS (http://lyh.pkmu.cn/ppus/), the first web server to predict PUS-specific Ψ sites. PPUS: employed support vector machine as the classifier and used nucleotides around Ψ sites as the features. Currently, PPUS: could accurately predict new Ψ sites for PUS1, PUS4 and PUS7 in yeast and PUS4 in human. PPUS: is well designed and friendly to user. AVAILABILITY AND IMPLEMENTATION: Our web server is available freely for non-commercial purposes at: http://lyh.pkmu.cn/ppus/ CONTACT: liyanhui@bjmu.edu.cn or cuiqinghua@hsc.pku.edu.cn.