Ping Zhou1, Jie Xia1, Yong-Jie Zhou1, Jun Wan1, Li Li1, Ji Bao1, Yu-Jun Shi1, Hong Bu1. 1. Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Abstract
AIM: To investigate whether the proportions of acetyl-histone-positive hepatocytes could be used as markers of deteriorating liver function. METHODS: In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically. RESULTS: The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05). CONCLUSION: The proportions of acetyl-histone-positive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.
AIM: To investigate whether the proportions of acetyl-histone-positive hepatocytes could be used as markers of deteriorating liver function. METHODS: In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically. RESULTS: The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05). CONCLUSION: The proportions of acetyl-histone-positive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.
Authors: Mohammad A Alsultan; Rashed S Alrshed; Abdulrahman A Aljumah; Salim A Baharoon; Yaseen M Arabi; Abdulaziz S Aldawood Journal: Saudi J Gastroenterol Date: 2011 Nov-Dec Impact factor: 2.485