Literature DB >> 26073660

Image analysis for accurately counting CD4+ and CD8+ T cells in human tissue.

Kurt Diem1, Amalia Magaret2, Alexis Klock3, Lei Jin3, Jia Zhu2, Lawrence Corey2.   

Abstract

In situ detection of specific cells offers a unique perspective on the spatial interactions between host immune cells and specific viral pathogens or cancers. Most immunohistochemistry techniques require manual cell counting on biopsied and fixed tissue sections. The availability of sophisticated software packages for analyzing fluorescently labeled tissue has made it possible to quickly and accurately quantitate the number of positive cells on such slides. Manual cell counting was compared to automatic cell counting using the program CellProfiler. The two techniques were used to count CD4+ and CD8+ T cells in human genital skin biopsies from herpesvirus type 2 (HSV-2) infected subjects. Manual counting and CellProfiler demonstrated high correlation both in cell counting as well as detection of immune cell "clustering" in tissue, an important visceral component of localized inflammation and characteristic of most chronic infections. Overall, CellProfiler is an effective and accurate method in addition to or replacement of manual cell counting of fluorescently labeled biopsies.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Cell counting; CellProfiler; HSV-2; Immunohistochemistry (IHC); Software cell counting

Mesh：

Year:  2015        PMID： 26073660      PMCID： PMC4558892          DOI： 10.1016/j.jviromet.2015.06.004

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  8 in total

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Authors:  Joshua T Schiffer; David A Swan; Lawrence Corey; Anna Wald
Journal:  Antimicrob Agents Chemother       Date:  2013-09-09       Impact factor: 5.191

2.  Biological imaging software tools.

Authors:  Kevin W Eliceiri; Michael R Berthold; Ilya G Goldberg; Luis Ibáñez; B S Manjunath; Maryann E Martone; Robert F Murphy; Hanchuan Peng; Anne L Plant; Badrinath Roysam; Nico Stuurman; Nico Stuurmann; Jason R Swedlow; Pavel Tomancak; Anne E Carpenter
Journal:  Nat Methods       Date:  2012-06-28       Impact factor: 28.547

Review 3.  Lymphoid tissue structure and HIV-1 infection: life or death for T cells.

Authors:  Ming Zeng; Ashley T Haase; Timothy W Schacker
Journal:  Trends Immunol       Date:  2012-05-19       Impact factor: 16.687

4.  Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivation.

Authors:  Jia Zhu; David M Koelle; Jianhong Cao; Julio Vazquez; Meei Li Huang; Florian Hladik; Anna Wald; Lawrence Corey
Journal:  J Exp Med       Date:  2007-02-26       Impact factor: 14.307

5.  Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisition.

Authors:  Jia Zhu; Florian Hladik; Amanda Woodward; Alexis Klock; Tao Peng; Christine Johnston; Michael Remington; Amalia Magaret; David M Koelle; Anna Wald; Lawrence Corey
Journal:  Nat Med       Date:  2009-08-02       Impact factor: 53.440

6.  Rapid localized spread and immunologic containment define Herpes simplex virus-2 reactivation in the human genital tract.

Authors:  Joshua T Schiffer; David Swan; Ramzi Al Sallaq; Amalia Magaret; Christine Johnston; Karen E Mark; Stacy Selke; Negusse Ocbamichael; Steve Kuntz; Jia Zhu; Barry Robinson; Meei-Li Huang; Keith R Jerome; Anna Wald; Lawrence Corey
Journal:  Elife       Date:  2013-04-16       Impact factor: 8.140

7.  CellProfiler: image analysis software for identifying and quantifying cell phenotypes.

Authors:  Anne E Carpenter; Thouis R Jones; Michael R Lamprecht; Colin Clarke; In Han Kang; Ola Friman; David A Guertin; Joo Han Chang; Robert A Lindquist; Jason Moffat; Polina Golland; David M Sabatini
Journal:  Genome Biol       Date:  2006-10-31       Impact factor: 13.583

8.  Location and dynamics of the immunodominant CD8 T cell response to SIVΔnef immunization and SIVmac251 vaginal challenge.

Authors:  Arun K Sasikala-Appukuttan; Hyeon O Kim; Nikilyn J Kinzel; Jung Joo Hong; Anthony J Smith; Reece Wagstaff; Cavan Reilly; Michael Piatak; Jeffrey D Lifson; R Keith Reeves; R Paul Johnson; Ashley T Haase; Pamela J Skinner
Journal:  PLoS One       Date:  2013-12-09       Impact factor: 3.240
  8 in total
  10 in total

1.  Recombinant Human Lactoferrin Reduces Inflammation and Increases Fluoroquinolone Penetration to Primary Granulomas During Mycobacterial Infection of C57Bl/6 Mice.

Authors:  Thao K T Nguyen; Zainab Niaz; Marian L Kruzel; Jeffrey K Actor
Journal:  Arch Immunol Ther Exp (Warsz)       Date:  2022-02-28       Impact factor: 4.291

2.  B cells, antibody-secreting cells, and virus-specific antibodies respond to herpes simplex virus 2 reactivation in skin.

Authors:  Emily S Ford; Anton M Sholukh; RuthMabel Boytz; Savanna S Carmack; Alexis Klock; Khamsone Phasouk; Danica Shao; Raabya Rossenkhan; Paul T Edlefsen; Tao Peng; Christine Johnston; Anna Wald; Jia Zhu; Lawrence Corey
Journal:  J Clin Invest       Date:  2021-05-03       Impact factor: 14.808

3.  Elevated Foxp3/CD8 Ratio in Lung Adenocarcinoma Metastatic Lymph Nodes Resected by Transcervical Extended Mediastinal Lymphadenectomy.

Authors:  Joanna Domagala-Kulawik; Iwona Kwiecien; Juliusz Pankowski; Monika Pasieka-Lis; Dominika Wolosz; Marcin Zielinski
Journal:  Biomed Res Int       Date:  2017-08-02       Impact factor: 3.411

4.  In vitro Cell Viability by CellProfiler® Software as Equivalent to MTT Assay.

Authors:  Luciana S Gasparini; Nayana D Macedo; Elisângela F Pimentel; Marcio Fronza; Valdemar L Junior; Warley S Borges; Eduardo R Cole; Tadeu U Andrade; Denise C Endringer; Dominik Lenz
Journal:  Pharmacogn Mag       Date:  2017-07-11       Impact factor: 1.085

5.  Systems pathology by multiplexed immunohistochemistry and whole-slide digital image analysis.

Authors:  Sami Blom; Lassi Paavolainen; Dmitrii Bychkov; Riku Turkki; Petra Mäki-Teeri; Annabrita Hemmes; Katja Välimäki; Johan Lundin; Olli Kallioniemi; Teijo Pellinen
Journal:  Sci Rep       Date:  2017-11-14       Impact factor: 4.379

6.  In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia.

Authors:  Chung H Dang; Martine Aubert; Harshana S De Silva Feelixge; Kurt Diem; Michelle A Loprieno; Pavitra Roychoudhury; Daniel Stone; Keith R Jerome
Journal:  Sci Rep       Date:  2017-04-19       Impact factor: 4.379

7.  Automated and Reproducible Detection of Vascular Endothelial Growth Factor (VEGF) in Renal Tissue Sections.

Authors:  Nayana Damiani Macedo; Aline Rodrigues Buzin; Isabela Bastos de Araujo; Breno Valentim Nogueira; Tadeu Uggere Andrade; Denise Coutinho Endringer; Dominik Lenz
Journal:  J Immunol Res       Date:  2019-03-19       Impact factor: 4.818

Review 8.  Challenges and Opportunities in the Statistical Analysis of Multiplex Immunofluorescence Data.

Authors:  Christopher M Wilson; Oscar E Ospina; Mary K Townsend; Jonathan Nguyen; Carlos Moran Segura; Joellen M Schildkraut; Shelley S Tworoger; Lauren C Peres; Brooke L Fridley
Journal:  Cancers (Basel)       Date:  2021-06-17       Impact factor: 6.575

Review 9.  Transcriptome meta-analysis reveals a dysregulation in extra cellular matrix and cell junction associated gene signatures during Dengue virus infection.

Authors:  Sumbul Afroz; Jeevan Giddaluru; Mohd Manzar Abbas; Nooruddin Khan
Journal:  Sci Rep       Date:  2016-09-21       Impact factor: 4.379

10.  No DCX-positive neurogenesis in the cerebral cortex of the adult primate.

Authors:  Ruo-Xu Liu; Jie Ma; Bin Wang; Tian Tian; Ning Guo; Shao-Jun Liu
Journal:  Neural Regen Res       Date:  2020-07       Impact factor: 5.135
  10 in total

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