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Literature DB >> 26072918

Effective Spatial Separation of PC12 and NIH3T3 Cells by the Microgrooved Surface of Biocompatible Polymer Substrates.

Huichang Gao¹, Hua Dong¹, Xiaodong Cao¹, Xiaoling Fu¹, Ye Zhu², Chuanbin Mao², Yingjun Wang¹.

Abstract

Most organs and tissues are composed of more than one type of cell that is spatially separated and located in different regions. This study used a microgrooved poly(lactic-co-glycolic acid) (PLGA) substrate to guide two types of cocultured cells to two spatially separated regions. Specifically, PC12 pheochromocytoma cells are guided to the inside of microgrooves, whereas NIH3T3 fibroblasts are guided to the ridge area in between neighboring parallel microgrooves. In addition, the microgrooved structures can significantly promote the proliferation and neural differentiation of PC12 cells as well as the osteogenic differentiation of NIH3T3 cells. Therefore, the microgrooved PLGA surface with separated PC12 and NIH3T3 cells can serve as a potential model system for studying nerve reconstruction in bone-repairing scaffolds.

Entities: CellLine Chemical Disease Gene Species

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Year: 2015 PMID： 26072918 PMCID： PMC4924521 DOI： 10.1021/acs.langmuir.5b01018

Source DB: PubMed Journal: Langmuir ISSN： 0743-7463 Impact factor: 3.882

51 in total

1. Micropatterning topology on soft substrates affects myoblast proliferation and differentiation.

Authors: Susi Zatti; Alice Zoso; Elena Serena; Camilla Luni; Elisa Cimetta; Nicola Elvassore
Journal: Langmuir Date: 2012-01-25 Impact factor: 3.882

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Authors: Uta Reich; Elena Fadeeva; Athanasia Warnecke; Gerrit Paasche; Peter Müller; Boris Chichkov; Timo Stöver; Thomas Lenarz; Günter Reuter
Journal: J Biomed Mater Res B Appl Biomater Date: 2012-01-30 Impact factor: 3.368

3. Observation of fibroblast motility on a micro-grooved hydrophobic elastomer substrate with different geometric characteristics.

Authors: Wen-Ta Su; Yung-Feng Liao; I-Ming Chu
Journal: Micron Date: 2006-05-15 Impact factor: 2.251

4. In vitro generation of osteochondral differentiation of human marrow mesenchymal stem cells in novel collagen-hydroxyapatite layered scaffolds.

Authors: Jiaan Zhou; Caixia Xu; Gang Wu; Xiaodong Cao; Liangming Zhang; Zhichen Zhai; Zhiwen Zheng; Xiaofeng Chen; Yingjun Wang
Journal: Acta Biomater Date: 2011-06-30 Impact factor: 8.947

5. Connective-tissue responses to defined biomaterial surfaces. II. Behavior of rat and mouse fibroblasts cultured on microgrooved substrates.

Authors: John C Grew; John L Ricci; Harold Alexander
Journal: J Biomed Mater Res A Date: 2008-05 Impact factor: 4.396

6. Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage.

Authors: Chor Yong Tay; Haiyang Yu; Mintu Pal; Wen Shing Leong; Nguan Soon Tan; Kee Woei Ng; David Tai Leong; Lay Poh Tan
Journal: Exp Cell Res Date: 2010-02-13 Impact factor: 3.905

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Authors: L Gonzalez-Mariscal; B Chávez de Ramírez; M Cereijido
Journal: J Membr Biol Date: 1985 Impact factor: 1.843

8. Mouse embryo-derived NIH3T3 fibroblasts adopt an osteoblast-like phenotype when treated with 1alpha,25-dihydroxyvitamin D(3) and dexamethasone in vitro.

Authors: Chaoxiang Shui; Andrew M Scutt
Journal: J Cell Physiol Date: 2002-11 Impact factor: 6.384

Effective Spatial Separation of PC12 and NIH3T3 Cells by the Microgrooved Surface of Biocompatible Polymer Substrates.

1. Micropatterning topology on soft substrates affects myoblast proliferation and differentiation.

2. Directing neuronal cell growth on implant material surfaces by microstructuring.

3. Observation of fibroblast motility on a micro-grooved hydrophobic elastomer substrate with different geometric characteristics.

4. In vitro generation of osteochondral differentiation of human marrow mesenchymal stem cells in novel collagen-hydroxyapatite layered scaffolds.

5. Connective-tissue responses to defined biomaterial surfaces. II. Behavior of rat and mouse fibroblasts cultured on microgrooved substrates.

6. Micropatterned matrix directs differentiation of human mesenchymal stem cells towards myocardial lineage.

7. Tight junction formation in cultured epithelial cells (MDCK).

8. Mouse embryo-derived NIH3T3 fibroblasts adopt an osteoblast-like phenotype when treated with 1alpha,25-dihydroxyvitamin D(3) and dexamethasone in vitro.

9. Hypoxia induces neurite outgrowth in PC12 cells that is mediated through adenosine A2A receptors.

10. Identification of novel regulators associated with early-phase osteoblast differentiation.

1. Multiplex Photoluminescent Silicon Nanoprobe for Diagnostic Bioimaging and Intracellular Analysis.

2. Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis.

3. Functionalized Stress Component onto Bio-template as a Pathway of Cytocompatibility.