An indirect asymmetrical sandwich ELISA using anti-allotype antibodies for the specific and quantitative measurement of mouse IgG2a of Igh-1b allotype.

We recently described an indirect double sandwich ELISA (Klein-Schneegans et al., J. Immunol. Methods (1989) 119, 117) which permits the specific and quantitative measurement of mouse IgM, IgA and IgG subclasses with one major exception: IgG2a of the b allotype (Igh-1b in mouse strains such as C57BL/6) could not be reliably quantitated even by a very specific and sensitive asymmetrical sandwich ELISA (using two different anti-IgG2a isotype antibodies for capture and for detection). We now describe a similar method based on the use of two different anti-IgG2a allotype antibodies for the capture and detection of IgG2a in the serum of Igh-1b mouse strains.