| Literature DB >> 26069019 |
Benedikt Wefers1, Melanie Meyer1,2, Svenja Hensler1,2, Sudeepta Panda1,2, Oskar Ortiz1, Wolfgang Wurst1,2,3,4, Ralf Kühn1,2.
Abstract
Gene targeting by sequence-specific nucleases in one-cell embryos provides an expedited mutagenesis approach in rodents. This technology has been recently established to create knockout and knockin mutants through sequence deletion or sequence insertion. This article provides protocols for the preparation and microinjection of nuclease mRNA and targeting vector DNA into fertilized mouse eggs. Furthermore, we provide guidelines for genotyping the desired mouse mutants. Curr. Protoc. Mouse Biol. 2:347-364 © 2012 by John Wiley & Sons, Inc.Entities:
Keywords: TAL nuclease; gene targeting; homologous recombination; mouse mutant; pronucleus injection; zinc-finger nuclease
Year: 2012 PMID： 26069019 DOI： 10.1002/9780470942390.mo120177
Source DB: PubMed Journal: Curr Protoc Mouse Biol ISSN： 2161-2617