Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores.

There is an error in the Correction published on May 15, 2015. The corrected headings for Fig 1B are “Cells stained with 1C1-A488” and “Cells stained with 1C1-A594”, not “Beads stained with 1C1-A488” and “Beads stained with 1C1-A594”. The publisher apologizes for the error. The correct text is:

Fig 1

Quenching by anti-Alexa Fluor mAbs.

(A) Fluorescence of Alexa Fluor 488 (A488) on microbeads coated with 1C1-A488 or PC-3 cells stained with 1C1-A488 was quenched with a titration of the benchmark, a rabbit anti-A488 polyclonal, or 1 of 3 anti-A488 mAbs. One representative experiment of multiple is shown. (B) Fluorescence of Alexa Fluor 594 (A594) on microbeads coated with 1C1-A594 or PC-3 cells stained with 1C1-A594 was quenched with a titration of 1 of 3 anti-A594 mAbs. One representative experiment of multiple is shown. (A, B) Median fluorescence intensities (MFIs) at each anti-A488 or anti-A594 mAb concentration were normalized against a buffer control. The chimeric IgG1 isotype control was used as a non-quenching mAb control. The IC50 values (microgram/ml) of quenching and the corresponding 95% confidence intervals (95% CI) are listed for both the microbead- and cell-based titrations.

There are a number of errors in the headings for Fig 1B, “Quenching by anti-Alexa Fluor mAbs.” “Beads coated with 1C1-A488” and “Cells stained with 1C1-A488” should be “Beads coated with 1C1-A594” and “Cells stained with 1C1-A594”. Please see the corrected Fig 1 here.