Elysia M S Tan1, Tinte Itinteang1, Daria A Chudakova1, Jonathan C Dunne1, Reginald Marsh2, Helen D Brasch1, Paul F Davis1, Swee T Tan3. 1. Gillies McIndoe Research Institute, Wellington, New Zealand. 2. Gillies McIndoe Research Institute, Wellington, New Zealand University of Auckland, Auckland, New Zealand. 3. Gillies McIndoe Research Institute, Wellington, New Zealand Centre for the Study & Treatment of Vascular Birthmarks, Wellington Regional Plastic, Maxillofacial & Burns Unit, Hutt Hospital, Wellington, New Zealand.
Abstract
AIMS: Interstitial CD45+ cells and T lymphocytes have previously been demonstrated within infantile haemangioma (IH). This study investigated the expression of B and T lymphocyte markers by the CD45+ population, and the expression of Thy-1, a marker of thymocyte progenitors, which have the ability to give rise to both B and T cells. METHODS: Immunohistochemical (IHC) staining was performed on proliferating and involuted IHs for the expression of CD45, CD3, CD20, CD79a, Thy-1 and CD34. The presence of mRNA corresponding to CD45, CD3G, CD20 and Thy-1 was confirmed by reverse transcriptase-polymerase chain reaction in snap-frozen IH tissues. Cell counting of 3,3-diaminobenzidine IHC-stained slides was performed on CD45+ only cells and dually stained CD45+/CD3+ cells or CD45+/CD20+ cells and analysed statistically. In situ hybridisation and mass spectrometry were also performed to confirm the presence and abundance of Thy-1, respectively. RESULTS: IHC staining showed a subpopulation of CD45+ interstitial cells that expressed the T lymphocyte marker, CD3, and another subpopulation that expressed the B lymphocyte marker, CD20, in proliferating and diminished in involuted IHs. The abundant expression of Thy-1 on the endothelium of proliferating, but not involuted IH, was demonstrated by IHC staining and confirmed by in situ hybridisation and mass spectrometry. CONCLUSIONS: Both B and T lymphocytes are present within the interstitium of proliferating and involuted IH. The expression of Thy-1 by the endothelium suggests that B and T cells in IH may have originated from within the lesion, rather than migrating from the peripheral circulation. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.
AIMS: Interstitial CD45+ cells and T lymphocytes have previously been demonstrated within infantile haemangioma (IH). This study investigated the expression of B and T lymphocyte markers by the CD45+ population, and the expression of Thy-1, a marker of thymocyte progenitors, which have the ability to give rise to both B and T cells. METHODS: Immunohistochemical (IHC) staining was performed on proliferating and involuted IHs for the expression of CD45, CD3, CD20, CD79a, Thy-1 and CD34. The presence of mRNA corresponding to CD45, CD3G, CD20 and Thy-1 was confirmed by reverse transcriptase-polymerase chain reaction in snap-frozen IH tissues. Cell counting of 3,3-diaminobenzidine IHC-stained slides was performed on CD45+ only cells and dually stained CD45+/CD3+ cells or CD45+/CD20+ cells and analysed statistically. In situ hybridisation and mass spectrometry were also performed to confirm the presence and abundance of Thy-1, respectively. RESULTS: IHC staining showed a subpopulation of CD45+ interstitial cells that expressed the T lymphocyte marker, CD3, and another subpopulation that expressed the B lymphocyte marker, CD20, in proliferating and diminished in involuted IHs. The abundant expression of Thy-1 on the endothelium of proliferating, but not involuted IH, was demonstrated by IHC staining and confirmed by in situ hybridisation and mass spectrometry. CONCLUSIONS: Both B and T lymphocytes are present within the interstitium of proliferating and involuted IH. The expression of Thy-1 by the endothelium suggests that B and T cells in IH may have originated from within the lesion, rather than migrating from the peripheral circulation. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.
Authors: Helen H Yu; Therese Featherston; Swee T Tan; Alice M Chibnall; Helen D Brasch; Paul F Davis; Tinte Itinteang Journal: Front Surg Date: 2016-08-02
Authors: Tinte Itinteang; Alice M Chibnall; Reginald Marsh; Jonathan C Dunne; Sophie de Jong; Paul F Davis; Philip Leadbitter; Swee T Tan Journal: Front Surg Date: 2016-02-09
Authors: Hugo N Humphries; Susrutha K Wickremesekera; Reginald W Marsh; Helen D Brasch; Shreeja Mehrotra; Swee T Tan; Tinte Itinteang Journal: Front Surg Date: 2018-01-22
Authors: Therese Featherston; Reginald Walter Marsh; Bede van Schaijik; Helen D Brasch; Swee T Tan; Tinte Itinteang Journal: Front Med (Lausanne) Date: 2017-07-20
Authors: Claire S Luke Krishnan; Helen D Brasch; Josie Patel; Nicholas Bockett; Erin Paterson; Paul F Davis; Swee T Tan Journal: Front Surg Date: 2021-03-19