Mosayeb Rostamian1, Saeed Sohrabi2, Hanie Kavosifard3, Hamid M Niknam4. 1. Immunology Department, Pasteur Institute of Iran, Tehran, Iran. 2. Department of Microbiology, Qom Branch of Islamic Azad University, Qom, Iran. 3. Department of Microbiology, Kurdistan Science and Research Branch, Islamic Azad University, Sanandaj, Iran. 4. Immunology Department, Pasteur Institute of Iran, Tehran, Iran. Electronic address: mahmoudzadehh@yahoo.com.
Abstract
BACKGROUND/ PURPOSE: Leishmania (L.) tropica is the causative agent of different forms of human leishmaniasis. There is little information about the role of Leishmania-specific antibodies in the immune response against L. tropica infection. The aim of this study is to evaluate the role of Leishmania-specific antibodies and their immunoglobulin G (IgG) isotypes in L. tropica infection. METHODS: L. tropica at two different doses (high dose, 106 parasites/mouse and low dose, 103 parasites/mouse) were used for infection of BALB/c mice. BALB/c mice infected with Leishmania major were used for comparison. Anti-Leishmania antibodies of the IgG1 and IgG2a isotypes were assayed by enzyme-linked immunosorbent assay. RESULTS: Our data showed that (1) a higher parasite dose results in higher levels of antibody. (2) L. tropica infection results in a lower IgG1 antibody response, compared with L. major infection. (3) The IgG2a/IgG1 antibody response in L. tropica infection is higher than that in L. major infection. CONCLUSION: A higher IgG2a/IgG1 ratio is associated with protective immune response in L. tropica infection. These data can help to approach the complex profile of immunity against L. tropica infection.
BACKGROUND/ PURPOSE:Leishmania (L.) tropica is the causative agent of different forms of humanleishmaniasis. There is little information about the role of Leishmania-specific antibodies in the immune response against L. tropicainfection. The aim of this study is to evaluate the role of Leishmania-specific antibodies and their immunoglobulin G (IgG) isotypes in L. tropicainfection. METHODS:L. tropica at two different doses (high dose, 106 parasites/mouse and low dose, 103 parasites/mouse) were used for infection of BALB/c mice. BALB/c mice infected with Leishmania major were used for comparison. Anti-Leishmania antibodies of the IgG1 and IgG2a isotypes were assayed by enzyme-linked immunosorbent assay. RESULTS: Our data showed that (1) a higher parasite dose results in higher levels of antibody. (2) L. tropicainfection results in a lower IgG1 antibody response, compared with L. majorinfection. (3) The IgG2a/IgG1 antibody response in L. tropicainfection is higher than that in L. majorinfection. CONCLUSION: A higher IgG2a/IgG1 ratio is associated with protective immune response in L. tropicainfection. These data can help to approach the complex profile of immunity against L. tropicainfection.
Authors: Katherine L Mallory; Justin A Taylor; Xiaoyan Zou; Ishita N Waghela; Cosette G Schneider; Michael Q Sibilo; Neeraja M Punde; Leah C Perazzo; Tatyana Savransky; Martha Sedegah; Sheetij Dutta; Chris J Janse; Norbert Pardi; Paulo J C Lin; Ying K Tam; Drew Weissman; Evelina Angov Journal: NPJ Vaccines Date: 2021-06-18 Impact factor: 7.344
Authors: Alexander K Andrianov; Alexander Marin; Ruixue Wang; Ananda Chowdhury; Pragati Agnihotri; Abdul S Yunus; Brian G Pierce; Roy A Mariuzza; Thomas R Fuerst Journal: Mol Pharm Date: 2020-06-23 Impact factor: 4.939