Huihui Zhang1, Xi Yang1, Chengzhang Li1, Shuhuan Shang1, Jiaxi Wang1. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China ; Department of Periodontology, School and Hospital of Stomatology, Wuhan University Wuhan 430079, Hubei Province, China.
Abstract
OBJECTIVES: This study was aimed to investigate effects of 2% minocycline-HCl paste root conditioning on periodontal surgery. MATERIALS AND METHODS: In vitro, cementum slices affected by periodontitis were randomly conditioned with 2% minocycline-HCl paste, 2% minocycline-HCl liquid, and 0.9% saline. NIH3T3 cells were cultured and attached to the each slide, and the viability and proliferation of cells were observed; In vivo, 21 deep periodontal pockets were treated by periodontal surgery, and the exposed root surfaces were randomly conditioned with 2% minocycline-HCl paste or 0.9% saline. The periodontal parameters were measured at baseline, after 3 and 6 months of periodontal surgery. RESULTS: In vitro, NIH3T3 cell showed better viability and proliferation at 3, 5, and 7 day in groups conditioned with minocycline-HCl than the group conditioned with 0.9% saline (at 3 day (P < 0.05); at 7 day (P < 0.01)) Minimal differences were found between minocycline-HCl paste and liquid groups; In vivo, 3 months after periodontal surgery, the greater CAL reduction was found in the minocycline-HCl treated group than in the control group (P < 0.05). The similar results were found for both CAL and PD (P < 0.05; P < 0.05) between two groups at 6 months after surgery. PI and SBI variations showed no statistical differences between two groups after periodontal surgery. CONCLUSION: Our results suggested that root conditioning with minocycline-HCl paste during periodontal surgery improve the periodontal healing, which may be associated with the promotion of the periodontal cell attachment and growth onto the root surfaces.
OBJECTIVES: This study was aimed to investigate effects of 2% minocycline-HCl paste root conditioning on periodontal surgery. MATERIALS AND METHODS: In vitro, cementum slices affected by periodontitis were randomly conditioned with 2% minocycline-HCl paste, 2% minocycline-HCl liquid, and 0.9% saline. NIH3T3 cells were cultured and attached to the each slide, and the viability and proliferation of cells were observed; In vivo, 21 deep periodontal pockets were treated by periodontal surgery, and the exposed root surfaces were randomly conditioned with 2% minocycline-HCl paste or 0.9% saline. The periodontal parameters were measured at baseline, after 3 and 6 months of periodontal surgery. RESULTS: In vitro, NIH3T3 cell showed better viability and proliferation at 3, 5, and 7 day in groups conditioned with minocycline-HCl than the group conditioned with 0.9% saline (at 3 day (P < 0.05); at 7 day (P < 0.01)) Minimal differences were found between minocycline-HCl paste and liquid groups; In vivo, 3 months after periodontal surgery, the greater CAL reduction was found in the minocycline-HCl treated group than in the control group (P < 0.05). The similar results were found for both CAL and PD (P < 0.05; P < 0.05) between two groups at 6 months after surgery. PI and SBI variations showed no statistical differences between two groups after periodontal surgery. CONCLUSION: Our results suggested that root conditioning with minocycline-HCl paste during periodontal surgery improve the periodontal healing, which may be associated with the promotion of the periodontal cell attachment and growth onto the root surfaces.
Entities:
Keywords:
Root conditioning; minocycline-HCl; periodontal surgery
Authors: R A Greenwald; L M Golub; B Lavietes; N S Ramamurthy; B Gruber; R S Laskin; T F McNamara Journal: J Rheumatol Date: 1987-02 Impact factor: 4.666