Xiaoyu Wang1, Chen Li1, Qiaoqun Dai2. 1. Department of Pediatrics, Lishui University Lishui, Zhejiang, 323000, China. 2. Department of Pediatrics, Lishui Maternal and Child Health Hospital Lishui, Zhejiang, 323000, China.
Abstract
BACKGROUND: Cardiomyocyte hypoxia causes cardiac hypertrophy and other major myocardial injuries. We investigated the molecular mechanism of microRNA-26b (miR-26b) in regulating hypoxia-induced apoptosis in rat neonatal cardiomyocytes. METHODS: Neonatal rat cardiomyocytes was prepared in vitro and hypoxia was induced. Apoptotic cardiomyocytes were examined by TUNEL staining and the expression of miR-26b were monitored by qRT-PCR. The effect of mir-26b downregulation on hypoxia-induced apoptosis, or expression of PTEN in cardiomyocytes was monitored. PTEN was knocked down in cardiomyocytes by siRNA to further investigate its association with miR-26b. RESULTS: Hypoxia induced severe apoptosis and upregulated miR-26b in neonatal rat cardiomyocytes in vitro. Down-regulation of miR-26b markedly ameliorated hypoxia-induced apoptosis and up-regulated PTEN. Luciferase reporter assay confirmed PTEN was directly targeted by miR-26b, and knocking down PTEN reduced cytotoxicity induced by miR-26b upregulation. CONCLUSION: Downregulation of miR-26b protected cardiomyocytes from hypoxia-induced apoptosis, and the protective effect was very likely to be associated with PTEN regulation.
BACKGROUND: Cardiomyocyte hypoxia causes cardiac hypertrophy and other major myocardial injuries. We investigated the molecular mechanism of microRNA-26b (miR-26b) in regulating hypoxia-induced apoptosis in rat neonatal cardiomyocytes. METHODS: Neonatal rat cardiomyocytes was prepared in vitro and hypoxia was induced. Apoptotic cardiomyocytes were examined by TUNEL staining and the expression of miR-26b were monitored by qRT-PCR. The effect of mir-26b downregulation on hypoxia-induced apoptosis, or expression of PTEN in cardiomyocytes was monitored. PTEN was knocked down in cardiomyocytes by siRNA to further investigate its association with miR-26b. RESULTS:Hypoxia induced severe apoptosis and upregulated miR-26b in neonatal rat cardiomyocytes in vitro. Down-regulation of miR-26b markedly ameliorated hypoxia-induced apoptosis and up-regulated PTEN. Luciferase reporter assay confirmed PTEN was directly targeted by miR-26b, and knocking down PTEN reduced cytotoxicity induced by miR-26b upregulation. CONCLUSION: Downregulation of miR-26b protected cardiomyocytes from hypoxia-induced apoptosis, and the protective effect was very likely to be associated with PTEN regulation.
Authors: Alan S Go; Dariush Mozaffarian; Véronique L Roger; Emelia J Benjamin; Jarett D Berry; Michael J Blaha; Shifan Dai; Earl S Ford; Caroline S Fox; Sheila Franco; Heather J Fullerton; Cathleen Gillespie; Susan M Hailpern; John A Heit; Virginia J Howard; Mark D Huffman; Suzanne E Judd; Brett M Kissela; Steven J Kittner; Daniel T Lackland; Judith H Lichtman; Lynda D Lisabeth; Rachel H Mackey; David J Magid; Gregory M Marcus; Ariane Marelli; David B Matchar; Darren K McGuire; Emile R Mohler; Claudia S Moy; Michael E Mussolino; Robert W Neumar; Graham Nichol; Dilip K Pandey; Nina P Paynter; Matthew J Reeves; Paul D Sorlie; Joel Stein; Amytis Towfighi; Tanya N Turan; Salim S Virani; Nathan D Wong; Daniel Woo; Melanie B Turner Journal: Circulation Date: 2013-12-18 Impact factor: 29.690
Authors: B Freude; T N Masters; F Robicsek; A Fokin; S Kostin; R Zimmermann; C Ullmann; S Lorenz-Meyer; J Schaper Journal: J Mol Cell Cardiol Date: 2000-02 Impact factor: 5.000