Fengsheng Li1, Dongping Ye1, Jun Duan1, Hao Sun1. 1. Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College, Jinan University Guangzhou 510220, P. R. China.
Abstract
OBJECTIVE: This study aims to explore the effect of osteopontin on the inflammatory reaction of cartilage cells stimulated by LPS and its possible signal pathway. METHODS: The inflammatory reaction of cartilage cells was induced by LPS, and then the cells were treated with OPN and PD98059 respectively. The expression of TNF-α, IL-1β and ERK1/2 were detected by ELISA or Western-blotting methods. RESULTS: Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS with dose dependent, while PD98059 could reverse the inhibition of osteopontin. CONCLUSION: Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS, which may be associated with the ERK1/2 signal pathway.
OBJECTIVE: This study aims to explore the effect of osteopontin on the inflammatory reaction of cartilage cells stimulated by LPS and its possible signal pathway. METHODS: The inflammatory reaction of cartilage cells was induced by LPS, and then the cells were treated with OPN and PD98059 respectively. The expression of TNF-α, IL-1β and ERK1/2 were detected by ELISA or Western-blotting methods. RESULTS:Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS with dose dependent, while PD98059 could reverse the inhibition of osteopontin. CONCLUSION:Osteopontin could decrease the cartilage cellular inflammatory reaction induced by LPS, which may be associated with the ERK1/2 signal pathway.
Entities:
Keywords:
ERK1/2 signal pathway; Osteopontin; inflammatory reaction
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