Shinichi Takaichi1, Takashi Maoka2. 1. Department of Biology, Nippon Medical School, Kyonan-cho 1, Musashino, 180-0023, Japan. takaichi@nms.ac.jp. 2. Research Institute for Production Development, Shimogamo-morimoto, Sakyo, Kyoto, 606-0805, Japan. maoka@mbox.kyoto-inet.or.jp.
Abstract
OBJECTIVES: Neurosporene is a carotene and an intermediate in the synthesis of lycopene from phytoene. Its content in carotenogenic organisms is very low; hence, the complete assignments of its spectroscopic data, including NMR, are insufficient. RESULTS: A purple bacterium of Rhodobacter sphaeroides G1C mutant had only one carotenoid. This carotenoid was extracted and purified using silica gel, DEAE-Toyopearl and C18-HPLC columns. It was identified using its absorption spectra, mass spectra, and (1)H- and (13)C-NMR spectra, including two-dimensional spectral analyses. CONCLUSION: The major carotenoid in the Rhodobacter sphaeroides G1C mutant was identified as neurosporene (7,8-dihydro-ψ,ψ-carotene) using spectroscopic measurements, including complete assignments of its (1)H- and (13)C-NMR spectral data.
OBJECTIVES:Neurosporene is a carotene and an intermediate in the synthesis of lycopene from phytoene. Its content in carotenogenic organisms is very low; hence, the complete assignments of its spectroscopic data, including NMR, are insufficient. RESULTS: A purple bacterium of Rhodobacter sphaeroidesG1C mutant had only one carotenoid. This carotenoid was extracted and purified using silica gel, DEAE-Toyopearl and C18-HPLC columns. It was identified using its absorption spectra, mass spectra, and (1)H- and (13)C-NMR spectra, including two-dimensional spectral analyses. CONCLUSION: The major carotenoid in the Rhodobacter sphaeroidesG1C mutant was identified as neurosporene (7,8-dihydro-ψ,ψ-carotene) using spectroscopic measurements, including complete assignments of its (1)H- and (13)C-NMR spectral data.
Authors: Piyush Behari Lal; Fritz M Wells; Yucai Lyu; Indro N Ghosh; Robert Landick; Patricia J Kiley Journal: Front Microbiol Date: 2019-10-11 Impact factor: 5.640
Authors: Maria Cristina P P Reis-Mansur; Janine S Cardoso-Rurr; Josemar V Maiworm Abreu Silva; Gabriela Rodrigues de Souza; Verônica da Silva Cardoso; Felipe Raposo Passos Mansoldo; Yuri Pinheiro; Júnia Schultz; Luciene B Lopez Balottin; Antonio Jorge Ribeiro da Silva; Claudia Lage; Elisabete Pereira Dos Santos; Alexandre Soares Rosado; Alane Beatriz Vermelho Journal: Sci Rep Date: 2019-07-02 Impact factor: 4.379