Mahdi Abastabar1, Ali Rezaei-Matehkolaei2, Mohammad Reza Shidfar1, Parivash Kordbacheh1, Rasoul Mohammadi3, Tahereh Shokoohi4, Mohammad Taghi Hedayati4, Nilufar Jalalizand1, Hossein Mirhendi1. 1. 1. Dept. of Medical Parasitology and Mycology, School of Public Health; National Institute of Health Research, Tehran University of Medical Sciences , Tehran, Iran. 2. 2. Dept. of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences , Ahvaz, Iran. 3. 3. Dept. of Medical Parasitology and Mycology, Faculty of Medicine, Isfahan University of Medical Sciences , Isfahan, Iran. 4. 4. Invasive Fungi Research Center(IFRC) and Dept. of Medical Mycology and Parasitology, Faculty of Medicine, Mazandaran University of Medical Sciences , Sari , Iran.
Abstract
BACKGROUND: Surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. In this study beta tubulin gene (BT2), was used as the first time in a PCR-RFLP format to clarify the distribution of dermatophytosis agents in some parts of Iran. METHODS: A total of 603 clinical isolates was obtained from 500 patients in Tehran, Isfahan, Mazandaran and Guilan provinces. The isolates were identified using macro/micro-morphological criteria and electrophoretic patterns of PCR amplicons of BT2after digestion with each of the restriction enzymes FatI, HpyCH4V, MwoI and Alw21I. RESULTS: Among the patients, 59.2% were male and 40.8% female. The most prevalent clinical form was tinea pedis (42.4%), followed by tinea cruris (24.2%), tinea unguium (12.3%), tinea corporis (10.8%), tinea faciei (4%), tinea manuum (3.14%), tinea capitis (3%) and tinea barbae (0.16%), respectively. Trichophyton interdigitale ranked the first, followed by T. rubrum, Epidermophyton floccosum, Microsporum canis, T. tonsurans, T. erinacei and T. violaceum (each 0.49%) and the less frequent species were T. schoenleinii, M. gypseum and T.anamorph of Arthroderma benhamiae (each 0.16%). A case of scalp infection by E. floccosum was an exceptional event in the study. No case of T. verrucosum was found. CONCLUSION: Trichophyton species and E. floccosum are yet the predominant agents of infection in Iran, while Microsporum species are decreasing. T. interdigitale and Tinea pedis remain as the most causal agent and clinical form of dermatophytosis, respectively. It seems that BT2 can be a useful genetic marker for epidemiological survey of common pathogenic dermatophytes.
BACKGROUND: Surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. In this study beta tubulin gene (BT2), was used as the first time in a PCR-RFLP format to clarify the distribution of dermatophytosis agents in some parts of Iran. METHODS: A total of 603 clinical isolates was obtained from 500 patients in Tehran, Isfahan, Mazandaran and Guilan provinces. The isolates were identified using macro/micro-morphological criteria and electrophoretic patterns of PCR amplicons of BT2after digestion with each of the restriction enzymes FatI, HpyCH4V, MwoI and Alw21I. RESULTS: Among the patients, 59.2% were male and 40.8% female. The most prevalent clinical form was tinea pedis (42.4%), followed by tinea cruris (24.2%), tinea unguium (12.3%), tinea corporis (10.8%), tinea faciei (4%), tinea manuum (3.14%), tinea capitis (3%) and tinea barbae (0.16%), respectively. Trichophyton interdigitale ranked the first, followed by T. rubrum, Epidermophyton floccosum, Microsporum canis, T. tonsurans, T. erinacei and T. violaceum (each 0.49%) and the less frequent species were T. schoenleinii, M. gypseum and T.anamorph of Arthroderma benhamiae (each 0.16%). A case of scalp infection by E. floccosum was an exceptional event in the study. No case of T. verrucosum was found. CONCLUSION: Trichophyton species and E. floccosum are yet the predominant agents of infection in Iran, while Microsporum species are decreasing. T. interdigitale and Tinea pedis remain as the most causal agent and clinical form of dermatophytosis, respectively. It seems that BT2 can be a useful genetic marker for epidemiological survey of common pathogenic dermatophytes.
Authors: K Makimura; Y Tamura; T Mochizuki; A Hasegawa; Y Tajiri; R Hanazawa; K Uchida; H Saito; H Yamaguchi Journal: J Clin Microbiol Date: 1999-04 Impact factor: 5.948
Authors: A Rezaei-Matehkolaei; K Makimura; Mr Shidfar; F Zaini; Mr Eshraghian; N Jalalizand; S Nouripour-Sisakht; L Hosseinpour; H Mirhendi Journal: Iran J Public Health Date: 2012-03-31 Impact factor: 1.429