Chengcheng Gao1, Tianheng Ma1, Liqun Pang1, Rui Xie1. 1. Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, P. R. China.
Abstract
BACKGROUND: Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids. OBJECTIVE: To investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids. METHODS: The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS. RESULTS: Initial screening showed that 100 µM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuronidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour. CONCLUSION: All these data indicated the possibility of cryptotanshinone's influence towards bile acids metabolism and homeostasis of bile acids.
BACKGROUND: Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids. OBJECTIVE: To investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids. METHODS: The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS. RESULTS: Initial screening showed that 100 µM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuronidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour. CONCLUSION: All these data indicated the possibility of cryptotanshinone's influence towards bile acids metabolism and homeostasis of bile acids.
Authors: Thomas J Erichsen; André Aehlen; Ursula Ehmer; Sandra Kalthoff; Michael P Manns; Christian P Strassburg Journal: J Hepatol Date: 2010-02-04 Impact factor: 25.083