Wei-Ping Li1, Yu-Feng Wang2, Jian Gao3, Ming-Lian Yu4, Yan-Yang Yu5, Yuan-Qing Yao1. 1. Gynecologic Surgical department, The General Hospital of Chinese People's Liberation Army, China. 2. In patient department, The General Hospital of Chinese People's Liberation Army, China. 3. Gynecologic Surgical department, People's Hospital in Juye, Heze City, Shandong province, China. 4. Department of Pharmacy, The Military General Hospital of Beijing PLA, Beijing, 100700, China. 5. Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi, China.
Abstract
BACKGROUND: Humans can be frequently exposed to Bisphenol A (BPA) via multiple sources, and babies are considered to be the most sensitive group to exposure of BPA. AIMS: To investigate the inhibition potential of BPA towards human liver microsomes (HLMs)-catalyzed zidovudine (AZT) glucuronidation. MATERIALS AND METHODS: In vitro HLMs incubation system was used to investigate the inhibition potential of BPA towards AZT glucuronidation. Both Dixon and Lineweaver-Burk plots were employed to determine the inhibition kinetic type, and nonlinear repression was utilized to calculate the inhibition kinetic parameters (Ki). RESULTS: Concentration-dependent inhibition of BPA towards AZT glucuronidation was observed. Both Dixon and Lineweaver-Burk plots showed that BPA exerted competitive inhibition towards the glucuronidation of AZT, and nonlinear repression with competitive equation was used to calculate the Ki value to be 3.2 µM. CONCLUSION: Potential BPA-AZT interaction might occur when the patients administered with AZT is also exposed to BPA.
BACKGROUND:Humans can be frequently exposed to Bisphenol A (BPA) via multiple sources, and babies are considered to be the most sensitive group to exposure of BPA. AIMS: To investigate the inhibition potential of BPA towards human liver microsomes (HLMs)-catalyzed zidovudine (AZT) glucuronidation. MATERIALS AND METHODS: In vitro HLMs incubation system was used to investigate the inhibition potential of BPA towards AZT glucuronidation. Both Dixon and Lineweaver-Burk plots were employed to determine the inhibition kinetic type, and nonlinear repression was utilized to calculate the inhibition kinetic parameters (Ki). RESULTS: Concentration-dependent inhibition of BPA towards AZT glucuronidation was observed. Both Dixon and Lineweaver-Burk plots showed that BPA exerted competitive inhibition towards the glucuronidation of AZT, and nonlinear repression with competitive equation was used to calculate the Ki value to be 3.2 µM. CONCLUSION: Potential BPA-AZT interaction might occur when the patients administered with AZT is also exposed to BPA.
Entities:
Keywords:
Bisphenol A (BPA); human liver microsomes (HLMs); zidovudine (AZT)
Authors: Rebecca M Nachman; Stephen D Fox; W Christopher Golden; Erica Sibinga; Timothy D Veenstra; John D Groopman; Peter S J Lees Journal: J Pediatr Date: 2013-01-11 Impact factor: 4.406